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Maybe I'm thinking of a different article, but was that the Chinese one where they used TDZ on nodal segments? Never had much luck with nodal segments myself. For clean stuff- seeds that are still enveloped in plant material should be sterile- not that the seeds themselves are tough to...

Dead, dry cellulose will not be propagatable, no. For most plants, you want meristematic tissue- stuff that's growing the fastest, usually a growing tip of some sort- and that includes floral material. For most plants, this includes nodes; not cannabis, best as I know. The literature seems...

It's tough to see from the irregular texture of the glass, but from the first two images, it looks like you already have callus, along with 1-2 shoots. You were starting with leaf material, and not nodes, right? If this is the case, you probably have callus with shoots. Your contamination...

0.25 mg/mL TDZ is way too high. Do you mean a final concentration of 0.25 mg per liter?

But given that ecigs just vaporize the product- supposedly with no combustion- are there even any particles to clog stomata? I seem to recall the nicotine and other compounds are dissolved or suspended in glycerin or propylene glycol. But I do agree that stuff like that should be kept outside...

Nicotine (as nicotine sulfate) has been used for decades as a pesticide- the reason some plants produce the stuff, in fact, is to deter pests from feeding. It used to be bottled and sold as Black Leaf 40, no idea what products have it now. IIRC it's still used by "organic" farmers. Nicotine...

Many plants are sensitive to ethylene at the part per billion level. The precise symptoms depend upon the crop; carnations are pretty much ruined by 1-10 ppb ethylene. For cannabis, ethylene probably "reinforces" gender, pushing plants towards females. Colloidal silver is used to produce male...

The containers normally employed in tissue culture (Magenta boxes, Magenta B-caps for baby food jars) allow sufficient gas exchange when the containers are sealed with Micropore tape. Also note that rockwool cubes can be autoclaved along with liquid media- no agar needed.

Dropp is the commercial version of TDZ; it's used to defoliate cotton in the field. If you live in cotton country, it's all over the place. Several plant growth regulators are used commercially (Liberty, for example) in large quantities in a form that is vastly less expensive than the lab...

Also note for TDZ that the source matters. TDZ is chiral; the stuff you get from tissue culture suppliers should be optically pure, while Dropp (the commercial product) is racemic- a mixture of both optical enantiomers. So, you can use Dropp, but you have to use twice as much to get the same...

After reading this part of the Wikipedia page on marijuana and gender determination, I wondered about this part: Ainsworth reviews that treatment with auxin and ethylene have feminizing effects, and that treatment with cytokinins and gibberellins have masculinizing effects. Given that...

Peracetic acid is a combination of hydrogen peroxide and acetic acid; from Wikipedia: Peracetic acid arises upon treatment of acetic acid with hydrogen peroxide, the equilibrium constant being 0.37 at room temperature This means the reaction does not go to completion; there's some finished...

Even without the excipients, it's expensive; $6 for one pound of Bonide Stump-Out, versus about $1.41 a pound from hydro stores in quantity.

Not that I could release; someone else owns the data. This does not take into account any solvent that may have a greater affinity for the product- the hash oil- than for escaping into the atmosphere. I'm certain there's very little residue from evaporated butane; however, do we really know...

We use them in tissue culture as an organic source of nitrogen. However, it is an expensive way to provide this element, and not all plants require it; in fact, it doesn't seem to help except for some plants that truly detest ammoniacal nitrogen, and don't do well with nitrate.

At risk of spreading rumors, there are some who suggest it can survive autoclaving. That shouldn't be possible. But viruses aren't really "alive," and the structure of TbMV is so... sound that it can reform from its individual parts, like some sort of weird Terminator virus. From that, there are...

Alcohols work well on enveloped viruses- again, the sort of thing that afflict animals. However, TbMV is resistant to alcohol. "Biejernik confirmed that extracts from infected tobacco plants infected others, even after filtration, treatment with alcohol or formalin or time delays of up to 3...

Another possibility, yes. There's one big problem with Physan 20 (also sold as Consan 20, and other names): it is not virucidal. More precisely, it has never proven to kill viruses that afflict plants; there was one paper from the 1950s, published casually, concerning the use of quaternary...

There is a very simple possible explanation: your plants are virused, and root aphids have absolutely nothing to do with it. Very little is known of the viruses afflicting the Cannabaceae. Worse, same as anything else concerning viruses, there's a lot of lore and broscience, and not a lot of...

That's just broscience. There's no "resetting" DNA through tissue culture.