dire wolf
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Clone porn
Bud Goodman's Perpetual Thread of Perpetually Good Buds, Man.
romulangenetics
2nd Generation
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Macro
BudGoodman
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I dunno, IME, given proper care and time, even the gangliest(?) get with the program... However, taking cuts from newer growth is the ticket to cuts that thrive in a hurry.Here's some stuff in this vein; My guess is the latter, the donor cells are different.... The first part could be in flux with some of what I describe above, so it's perhaps a little of both.
Scientists have known for some time that 'clonal' (regenerant) organisms are not always identical: their observable characteristics and traits can vary, and this variation can be passed on to the next generation. This is despite the fact that they are derived from genetically identical founder cells.
They found that observable variations in regenerant plants are substantially due to high frequencies of mutations in the DNA sequence of these regenerants, mutations which are not contained in the genome of the parent plant.
'Where these new mutations actually come from is still a mystery,' said Professor Harberd. 'They may arise during the regeneration process itself or during the cell divisions in the donor plant that gave rise to the root cells from which the regenerant plants are created. We are planning further research to find out which of these two processes is responsible for these mutations. What we can say is that Nature has safely been employing what you might call a 'cloning' process in plants for millions of years, and that there must be good evolutionary reasons why these mutations are introduced.'
Why plant 'clones' aren't identical
A new study of plants that are reproduced by 'cloning' has shown why cloned plants are not identical. Scientists have known for some time that 'clonal' (regenerant) organisms are not always identical. Now researchers believe they have found out why this is the case in plants: the genomes of...www.sciencedaily.com
Monozygotic (identical) twins are natural clones. Clones contain identical sets of genetic material in the nucleus—the compartment that contains the chromosomes—of every cell in their bodies. Thus, cells from two clones have the same DNA and the same genes in their nuclei.
Monozygotic twins form from a single zygote that divides into two separate cell masses, each giving rise to an independent individual.
So in my mind, that means only true "clones" come from what they call tissue cultures. Wouldn't that be cool to lift this off the ground as a tool bud? What do you think? It's not rocket science. lol
Tissue Culture Propagation vs. Cloning - GrowerIQ.ca
Tissue culture propagation or traditional cloning - what's the difference and which method is right for you? Shlomo Booklin dives into the pros and cons.groweriq.ca
A Brief Introduction to Cannabis Tissue Culture and Micropropagation
Traditional cloning is so 2017hightimes.com
Tissue culture interests me... But moreso for vaulting things long(er) term... Maintaining eighty cultivars in a cloning cycle, when you're only flowering a dozen (or so) at a time, is kinda ridiculous.
Plug boiler.
Lovely... I use a magnifier app, on my phone, to try to get those kind of shots... But they just don't compare to a good camera and lens.
BudGoodman
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These are just the plants that I have in soil.
I have others, in rooters, that are not on that list.
I culled 32 a couple of days ago...
I have others, in rooters, that are not on that list.
I culled 32 a couple of days ago...
Trustfall
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The purge gets me everytime. I always do it mid flower then I have the best finish of the purged strain I can’t get back
dire wolf
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Hey Bud , you write on both sides of the page ?
Yet you won't split or boil your rapid rooters ......very suspicious
GrowHobo
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I think it’s time for an intervention.
BudGoodman
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Hey Bud , you write on both sides of the page ?
Yet you won't split or boil your rapid rooters ......very suspicious
BudGoodman
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That's another reason it's important to share things.The purge gets me everytime. I always do it mid flower then I have the best finish of the purged strain I can’t get back
thejack
- 67
- 33
thejack
- 67
- 33
Mr Budman, those baby plants look, I mean looked, great. While I'm not familiar with disco funk and mad dawg (chemdog cross?) I'm familiar with, but never grew Guava.
However, I am not only familiar with Chemdog, but LOVE & CHERISH the old Chemdog numbers. Jebus, for this old fart, it is my top of the pops type weed. But that's just how I respond to it.
Ever hear of "Permafrost"? Had that Lysol taste I pine for....get it? Pine for. There was good strains going around back then. Buthell,there's always good strains going around, 'eh? But nothing like Butterscotch Hawaiian X G13. I miss old breeders. Anyway, it would take a looooong time to read all your posts. So I prolly won't. I WILL be watching, though. One question please: what do you think about Autos? No hurry,take your time,and thanx.. thejack. '
thejack
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Looks like plants wearing shoes ; /
Trustfall
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I’ve felt the same but after seeing your generosity it’s upped my generosity as well. You never know, someone’s life you change today might come back and change your life tomorrow. Generosity can go a long way.That's another reason it's important to share things.
GrowHobo
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Together maybe we can all defeat corporate/commercial weed
Frankster
Never trust a doctor who's plants have died.
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Good, I'll get on it and start investigating it more toughly, I just wanted to get your thoughts on it first. As long as we got the specimens, then it should be a go, I can't see any issues. Like you said, having the optimum health parameters and whatnot in line is of paramount importance, cause preserving new growth in an optimum state is ideal, I would think.I dunno, IME, given proper care and time, even the gangliest(?) get with the program... However, taking cuts from newer growth is the ticket to cuts that thrive in a hurry.
Tissue culture interests me... But moreso for vaulting things long(er) term... Maintaining eighty cultivars in a cloning cycle, when you're only flowering a dozen (or so) at a time, is kinda ridiculous.
Plug boiler.
Lovely... I use a magnifier app, on my phone, to try to get those kind of shots... But they just don't compare to a good camera and lens.
So that will likely entail getting a system in place here, perhaps, first; then getting that stuff out too you, with any support you need to make it into a reality. I know we can do this; I'm quite sure of it. It's just getting all the pH, and some bio co-factors, a few enzymes, and bits of energy in vitro. easy peasy. (already have test tubes, need a stand) The biggest part, perhaps is having vigor, prolific hardiness built forward, going into the process. Not only getting them to take, but also effecting overall quality, and shelf life. Plus the environmental conditions, that's going to be a big factor also.
Dr. Frankenstein's Ganja biomolecular archive film. lol
This sounds like a fun challenge.
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Frankster
Never trust a doctor who's plants have died.
Supporter
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That's a big fact Trustfall. You just never know. Weird things happen bro, and everyone doesn't look at the world the same way.
This thread really made my day.
The farm reminds me of the federation; full of all sorts of different lifeforms, that (usually) get along.Together maybe we can all defeat corporate/commercial weed
That's a tall order, but certainly a worthwhile endeavor; if you can find like minded individuals.
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Frankster
Never trust a doctor who's plants have died.
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A really good thread to mark; Really cool stuff Maynard 
I like pushing buttons.
I like pushing buttons.
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Frankster
Never trust a doctor who's plants have died.
Supporter
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Just about how I look at things these day, it usually depends on how I'm feeling at any given moment. Laugh, or help, or both.
But, if your waiting on the SPD, I say, don't hold your breath too long...
But, if your waiting on the SPD, I say, don't hold your breath too long...
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GrowHobo
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All is say was lol for the longest time.Just about how I look at things these day, it usually depends on how I'm feeling at any given moment. Laugh, or help, or both.
But, if your waiting on the SPD, I say, don't hold your breath too long...
View attachment 1177735
Frankster
Never trust a doctor who's plants have died.
Supporter
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Wowza, this is perhaps, one of the best ways to approach this I think; Difficult to believe I can get one of these at this price.
Anyone want to take a stab at what this thing does? I've spent many hours/years slaving around these things. Was part of my gig for a long time.
Anyone want to take a stab at what this thing does?
I've spent many hours/years slaving around these things. Was part of my gig for a long time.
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