Best practice is trial/error.....Perseverance preceed success!!
Whats your method?
I do like to peel the part of the stem that goes in the medium or below the disc if you are aerocloning....i noticed that the callus is forming huge and from it an explosion of roots is originated...
I think that the most important part is the environment...humid enough to dont stress the plant but with enough transpiration to create a very slow dryback that creates the need of roots....
Pretty much also in aereocloning, once you nail it (Good Environment) you can chop cuts with your fingernails and shove em in anything and they will root!!!
In my experience i stopped to do two stupid things like starve of nitrogen the cut to encourage roots formation (Now i feed em like regular veggin plants plus
superthrive and they look great)
and soak the cut in water for 12 to 24 hours (it lead me to many cuts gettin some sort of fungus and either die or just took off more slowly)
Ive never been sterile too...