How do you select a male for breeding??

[KING_KONG_KOLAS]

How do you select a male for breeding if the male in question has never been bred before. If the males have not been bred befor, what do you look for in male for breeding stock?

 

[astonyx]

stature of its grow, how big its flowers are, vigour, some resin , nice smell many things depends what you are trying to get.

 

[strainman]

Depends on what traits you are breeding for and trying to stabilize but like astonyx said height, shape, smell, looks, vigour etc...

 

[Skunkenstein]

If you are Familiar with the strain and have several Males to work with..Treat them like you would a Female plant.Once they have sexed 'Naturally',put them under a 12/12 Light schedule and Observe all pertinant characteristics.Structure,node spacing,vigor,smell,resin production.The 'Stud' male will usually standout from the rest!!A male plant that actually produces standing Trichromes is a very good Indicator in my Book.Males always tend to be Taller and sex sooner than girls..but not always.I will not use the first or last male that shows sex..usually shoot for the Middle.The Last Breeding project I was Involved with, the chosen Male plant was more Exciting than the Girls he was Bred too..!! He had all the special Traits you would want..!!

 

[KING_KONG_KOLAS]

thanks guys.. exactly the kind of info I was looking for.

 

[supasticky]

Inner-node spacing, Color, Resin production (if any), and size. Basically anything that sets one apart from the rest that is a quality you want to keep.

-Supa

 

[aireoponics]

or early traits/ autoflower traits, alot of people starting to breed autoflowers

 

[Dirty White Boy]

Man all the breeding with autoflowering crap, an S1's is sooooo detrimintal to future lines, sigh. Just stop it. As far as males you can deff. look for signs of a healthy plant, but its IMPOSSIBLE to know what a male plant will do till you use it, make a cross test that cross. No short cuts, just lots of room time and skill. Or you can just pick the male who "sure does got a purty mouth". Lay the nearest female over and let him do his thing you sick sick pervert, lmao.

Otoe

 

[negative 1]

first thing you do is cross it to a female of desrired traits <this is all in my opinion> grow out the seeds from the resulting cross and see whats passed on to the offspring as far as traits you are looking for. as far as traits go it's what you like in a plant. wether it be smell, structure of flower formation, internodal spacing leaf size, whatever you get the picture. really the only way to know is to cross it and see what grows on with the offspring. if you like them, select again for charectaristics you find desireable and do a f2 cross of a brother and sister, grow these out and select once again for desireable charectaristics, cross these siblings for your f-3 generation, the process just keeps repeating until you get to where you want to be. this is a very tricky feat to pull off though i may add and all crossed plants should be kept for stabalization purposes or if you make a wrong selection somewhere in you crossings and need to start over you can start from that point and not from the beginning....so in one word a male should be selected CAREFULLY

 

[KING_KONG_KOLAS]

Ok say you do your fist cross with said male. And from the seeds you select a female of desired traits and then back cross that female to her fathers pollen. Then repeat the same process using the same pollen from the 1st cross. What kind of breeding technique is this called?

 

[KING_KONG_KOLAS]

Versus using sibling seeds m/f for your crosses?

 

[negative 1]

check this out

Inbred Lines and Lines Derived from Inbred Lines

Inbred strain: An Inbred strain is one that is homozygous at every locus and the alleles at each locus are identical by descent.

Inbred strains take 20 generations of brother-sister matings to achieve. Starting with a single ancestral breeding pair, a brother and sister are chosen each generation. Alternatively, inbred lines can be established by mating offspring back to a parent in a regular pattern. Occasionally, other systems of inbreeding might be used but a line cannot be considered inbred until it reaches an inbreeding coefficient equal to 20 generations of brother sister matings (99% or better). Inbred lines generations are often designated as F generations (F3, F10, F20 etc.)

Substrain: Two inbred lines from a common origin may be considered substrains. This implies that the substrains differ at several loci. If two inbred lines are separated before 40 generations of inbreeding, there will still be enough heterozygosity (less than 1% of the genes would vary) at separation that two genetically different substrains could result.

Alternatively, genetic variation between the branches may have occur by mutation and genetic drift. If the variation is at a single locus this would be a coisogenic line (see below). If it is at several loci, it is a substrain. Finally, if variation between isolated branches of an inbred line can be demonstrated (per haps my microsatellite analyses) they the branches can be considered substrains.


Recombinant inbred (RI) strains are formed by crossing two inbred strains to make an F1 generation. Brother and sister pairs from this F1 generation are used to establish many different inbred lines. These RI lines require 20 or more generations of brother-sister matings.

The RI lines are started from a limited genetic pool. The original inbred lines differed at a number of loci. The RI lines differ at an intermediate number of loci when compared to the progenitor strains.

RI strains are designated by an abbreviation of both parental strain names separated by a capital X. For example, CXB, are recombinant inbred strains derived from a cross of BALB/c x C57BL.

Congenic Strains: The idea is to obtain strains that differ by only a few specified genes (ideally by a single gene). Usually, two inbred lines are crossed to make an F1 generation (here, however, it will be called an N(1) - see below). The F1 progeny are backcrossed to one of the parental inbred lines. These N(2) progeny will now have 75% of their material derived from a single parental line. N(2) progeny are again backcrossed to the same parental line to make an N(3) generation, etc. It takes 10 generations of backcrossing for a line to be considered as congenic.

After 10 generations, the congenic line will differ from the parental line by the gene of interest and a short linked chromosomal segment around that gene.

Speed Congenics: It takes a minimum of 10 generations to get a congenic line. In mice, this is approximately 2.5 years. Speed congenics are an attempt to cut that time.

Speed Congenics are merely a variation on regular congenics. At generation N(2) individual mice (generally males) are genotyped for 60 to 100 microsatellite markers and the mice with the that have the greatest share of the inbred background chromosomes you are interested in are selected as parents. (Males are used since a single male can be backcrossed to many different Parental strain females). This processes may eventually be further accelerated by microarray screens.

The savings can be considerable. Speed congenic technology might give you a congenic strain in 12 to 18 months versus 2 to 3 years.

Coisogenic Strains: Two strains that are genetically identical (i.e. isogenic), except for a single locus. This occurs most often by a spontaneous mutations by many generations of backcrossing. Coisogenic strains are also becoming available due to target mutagenesis (knock-outs) in Embryonic Stem cells (ES). For example, a target mutation is induced in an inbred strain (e.g. 129SV) and the mutant mice are backcrossed repeatedly to the same inbred substrain from which the ES cells were derived would be a coisogenic line. but the possibility of mutations elsewhere should be considered. Similarly, chemically or radiation induced mutants in an inbred background can be considered coisogenic (but only if other mutations are not present).

Recombinant Congenic (RC) A combination of congenic and inbred lines. Two inbred lines are crossed to form an F1. The F1 progeny are backcrossed to one of the parent lines for Strains are formed by crossing two inbred strains, followed by a few (usually two) backcrosses to one of the parental strains (the "background" strain), with subsequent inbreeding without selection for specific. Such a strain is developed by crossing male C57BL/6J mice with BALB/c females, followed by repeated backcrossing of female offspring to male C57BL/6J.

As with congenic strains, a minimum of 10 backcross generations is required, counting the F1 generation as generation 1. For full inbred status, however, you need 20 inbred generation equivalents

One generation of backcrossing is equivalent to two generations of brother-sister matings. Thus, a strain with one backcross [N(2)] is equivalent to a strain with 4 generations of inbreeding F4. For full inbred status, it would require an addition 16 generations of brother sister matings.

Segregating inbred strains: are developed by inbreeding with but one or more loci must remain heterozygous. This generally requires progeny screening each generation and selection of heterozygous parents.

As with any inbred strains it takes 20 generations of brother sister mating. These lines are designated as FH lines to distinguish them from homozygous inbred lines.

Consomic strains are produced by repeated backcrossing of a whole chromosome such as the X or Y chromosome onto an inbred strain. As with congenic strains, a minimum of 10 backcross generations is required.

Two strains A and B are crossed. In the cross A x B the F1 male progeny would have their X chromosome from the A strain and the Y chromosome from the B strain. The autosomes are from both strains. With repeated backcrossing to the A you will obtain a line of animals that have all A-strain autosomes, A-strain X-chromosome and B strain Y-chromosome. Similar methods can be used to get X chromosomes into different backgrounds

The strains are designated as host stain - chromosome (X or Y) and the donor stain. In the example above, this strain would be A-YB

Conplastic strains are developed by backcrossing the nuclear genome from one strain into the cytoplasm of another, i.e. the mitochondrial parent is always the female parent during the backcrossing program.

Assuming that the cytoplasmic environment is determined mostly by genome DNA, conplastic strains separate mitochondria from cytoplasmic effects.

Mice from two inbred strains (A x B) are crossed to produce an F1 generation. This female will have mitochondria from the A strain and a cytoplasm environment unique to the A x B genotype. If this female is mated back to strain B, the N2 females will still have the mitochondria from strain A but her cytoplasm is becoming more B-like. After 10 generations of backcrossing the conplastic line will have almost all of the chromosomes from line B, the cytoplasmic environment from line B but the mitochondria from line A.