Very Very Nice obs! So are you saying that its more the utensils and tissue that need to be sterile? Do you do you culture's in a normal environment?
Sorry if i'm asking too many questions but this is something i'm very interested in, i have more questions but i'll give you a chance to answer those first.
Herer - i've been talking to my homie PGW about this for awhile, i think i'm gonna give it a go this month.
PGW - Hey bro after seeing Obs pics, i'm gonna pick up that kit! Sounds like the ultimate way to keep a mother imo! Hopefully you can take this for a whirl sometime soon as well. If anybody will master this it'll be you!
well as a whole you need to be steriel i can't say one step is less critical than another.
i don't have a fancy laminar flow hood i use a plastic tub layed on the side
everything gets sprayed with iso over and over , the utensils are denatured alcohol and then burned off before use and then dipped back in after every singe move and burned off again .
sterilizing the agar and jars is easy in an autoclave
another tip is to scrub your arms with a stiff bristle brush to remove as much dead skin a possible as this attracts dust mites.
dust mites are your biggest enemy in tissue culture and they feed on dead skin .
