The effects of GABA in cannabis plants

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Frankster

Frankster

Does anyone have any experience with using this??? From what I understand it helps with plant vigor and increases the immunity respronses in cannabis, increasing THC and especially CBD levels.

Alright, got me a big pile of 100% pure GABA and going to try it out.... First, the taste test... (Taste bitter and only activates taste buds on the far distal parts of the toungue, otherwise tasteless)

The non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues in response to biotic and abiotic stress, and regulates plant growth.

In plants, GABA is proposed to take multiple functions under non-stressed and stressed conditions. It is a key metabolite for primary and secondary pathways being an important intermediate of nitrogen metabolism and amino acid biosynthesis. In addition, the GABA metabolism through the GABA shunt provides a source for carbon skeletons and energy for down-stream biosynthetic pathways. GABA is also involved in signaling or regulatory mechanisms. It indirectly affects plant growth and development throughout the whole crop cycle and it accumulates rapidly in response to abiotic stresses. It has been shown to contribute to responses to biotic stresses through multiple mechanisms. An overactivation of the GABA shunt can help to restrict the spread of necrotrophic fungi like Botrytis. The activity of GABA against insects may be based either on a direct inhibitory effect or on an induction of down-stream defense reactions or on a combination of both mechanisms. The dual function of GABA as a metabolite and as a component of signaling pathways is a combination enabling plants to cope with different conditions. Exogenously applied GABA triggers similar effects than the intrinsic molecule and may, therefore, offer the potential to improve the overall vigor of plants.

It can be found in all types of organisms including bacteria, fungi, plants and animals
In addition, it seems to be an important component in the regulation of carbon: nitrogen metabolism

There is strong evidence that GABA is involved in mediating tolerance towards a variety of environmental stresses such as low light, salinity, nitrogen starvation, drought or temperature (Kinnersley & Turano, 2000) and that it promotes plant growth and mitigates stress via up-regulating antioxidant defense systems and may therefore also contribute to the improvement of shelf life and crop quality during storage. The GABA metabolism has been shown to be involved in the recycling and reallocation of nitrogen during leaf senescence caused by abiotic stress (Jalil, Ahmad, & Ansari, 2017). In addition, it was demonstrated that it can also play a role in the defense of plants towards biotic stress caused by insects and necrotrophic fungi
 
Frankster

Frankster

Did some foliars tonight, some discoveries so far. It's highly dissolvable in water, nothing else required. I started out using approx 1/16 tsp in 1000 ml (ppm says 25 in RO water) and washed both the plants down and the soil, as well. pH is around 6.5-7 from the looks of it.

This is what the index say's, it's actually got two different pH levels, which is a good thing, should help buffering effects.... dissolution into a carboxyl and amine in H20.

Although in chemical terms, GABA is an amino acid (as it has both a primary amine and a carboxylic acid functional group), it is rarely referred to as such. GABA is not an alpha amino acid, meaning the amino group is not attached to the alpha carbon so it is not incorporated into proteins.

Basically, this little molecule modulates multiple functions, not only within THC/CBD production and ratio's, but is also a precursor fractional component to all cellular functions, and a key component as a chemical messenger signaling between the plant, and it's benefical microbes.

A metabolic product of plants and microorganisms produced by the decarboxylation of glutamic acid.

Under normal conditions, Glu takes part in seed germination, root architecture, pollen germination, and pollen tube growth. Under stree, Glu participates in wound response, pathogen resistance, response and adaptation to abiotic stress (such as salt, cold, heat, and drought), and local stimulation (abiotic or biotic stress)-triggered long distance signaling transduction. Glu signaling in plants,

Properties
Chemical formulaC4H9NO2
Molar mass103.120 g/mol
Appearancewhite microcrystalline powder
Density1.11 g/mL
Melting point203.7 °C (398.7 °F; 476.8 K)
Boiling point247.9 °C (478.2 °F; 521.0 K)
Solubility in water130 g/100 mL
log P−3.17
Acidity (pKa)
  • 4.031 (carboxyl; H2O)
  • 10.556 (amino; H2O)
 
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Frankster

Frankster

curious if gaba changes the terpene profile. Is this your first time using it in your grow?
This is exactly what the research suggest actually, yes, it changes the profiles, and might serve as a means of application for wide scale commercial production. Increasing CBD content, and keeping stuff from popping out of flowering.... ect... it also supposed to be highly healthy for the plants in general.

@MIMedGrower Maybe, who knows? If it looks like it's something that's highly beneficial, or actually works, sure! I like having options, and I don't mind sharing information, and I'm certainly not trying to push anything on anyone, but I'm most certainly interested in chemical pre-cursors, hormones, cytokines, growth factors, ect... and/or anything else that has to do with the central mechanisms surrounding plant energy production, stress response and immunity, and chemical movers.

It's why I installed the T5 UV A-B spectrum lights also, because wide spectrums could well enhance processes such as these. Finding ways of natrually stressing the plant responses. Creating the full potential of a specific genetic expression, it needs the proper diet + light.

Fortunately, Most plant fractions are widely available in the supplement industry, and available in pure forms, it's not an approach most biologist would take, because it's expensive, and raising one plant to grow another isn't exactly a highly sustainable economic policy, but for high value crops that's changing.

I've found that certain things like Yukka extracts, Glycerin (a extract component) among others tend to be highly beneficial to growing. GABA is isolated from potato tubers, so probably has many root mechanisms as well as what's already discovered. Many of these ideas are up to date, cutting edge research, not something from 20 years ago.

This is not bullshit.

Highlights


Both salicylic acid and GABA induced THCAS gene expression.

There was a significant correlation between THCAS gene expression and THC content.

The higher amount of THC coincided with the lower amount of CBD.

Salicylic acid and GABA can be used as elicitor compounds to massively produce THC and CBD.

Standard protocol based on spraying on plants to elicit specific metabolites which are mainly produced at flowering time and in flowering organs has many benefits alongside other elicitation approaches.




And yes, I can quote dozens of other species of plant this approach works on as well. Looks like it works on peppers, and some other crops. it looks like it works across a wide variety of plant species actually, and is not isolated to being used for cannabis only.... Take a min, and read the article, then give me your thoughts. Instead of just mocking me.
 
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Frankster

Frankster

There are lot's of research being done in this area.


Here's the pepper article, I think it's all works from the same premise, actually.

Improved drought tolerance in grass... (something we already know for sure increases cannabis potency, during late flowering)
 
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Frankster

Frankster

@Frankster in no way did i mean to say you push anything on anyone. Experiment away my friend. Experiment away!
LOL,
Yea, I realize I might be a little renegade freethinker on some pretty obscure and somewhat esoteric approaches, and maybe that's not always going to pan out (it doesn't), but I think it can, and it certainly does work occasionally, because I've got a handful of highly effective approaches by trying random things, to see if it helps. Some of my ideas might be a little nutty, I suppose. As long as there's a decent theoretical chance, I'm willing to feed the plant most anything, then move on to the next idea. There's a ton of biochemicals fractions in plants available, so it's not like there' a shortage of things to try, and it's all relatively cheap and widely available. Usually your aware right away if your on the right track or not.

For me, it's fun, and something new to do. What I really need is more space, so I can expand and do more than just play around. I need a greenhouse.

The proof will be with these guys, if it works. They are light colored and small, but they have nice budding sites so far, by the looks of it. They've also been getting 12 full hours of UV light for awhile now, LOL, that's a LOT of stress..
 
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Arwood

Arwood

Good luck, you amazing scientist you. I might not be too far behind when I can figure out how to measure out the gaba correctly.
On that note, been reading another thread where aqua man is assisting another (I asked for help too) for a self watering system. Mixing that with different jugs of treated water would make for an excellent controlled experiment. At least I think so. Measuring out water in ball jars is getting old fast.

I really love the knowledge gained from this community. I hope I can give back even half as much as the rest of y’all.
 
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