Were you winterizing and doing a hexane/saline solution wash, before dissolving the iso extract in hexane for this column chromatography?
As a side note, thank you Graywolf for your positive influence on the internet. I’ve been reading different forums for years, and learned a lot from intelligent people such as yourself. This is my first time posting so I just wanted to take the opportunity to thank you.
We winterized, but didn't do a saline wash.
Thanks for the good thoughts brother! I believe that the forums are what we make them and what goes around comes around.
Acts of kindness don't melt the sociopaths heart, but they promote and encourage similar acts of kindness from the rest of us. Disharmonious acts sooner or later pretty much pisses everyone off, so their response is less likely to be kindly and in good humor as well.
One technique that helps me greatly is time. It allows JD, retired corporate warhorse, to morph into doppelganger Graywolf, who is a more nurturing kindly spirit, to reflect and edit my posts before hitting send. Sometimes my first response requires considerable editing.