• Home
  • Forums
  • Medical Cannabis Cultivation
  • Advanced Techniques & Problems
  • PTC - Micropropagation of Cannabis

PTC - Micropropagation of Cannabis

  • Thread starter Thread starter Cultiv8r
  • Start date Start date Jan 31, 2011
  • Tagged users Tagged users None

PTC - Micropropagation of Cannabis

Cultiv8r Jan 31, 2011 7 Replies 4,823 Views
Page 1 of 1 · Replies 1–8 of 8
1

Cultiv8r

Posts
25
Joined
Aug 20, 2010
Points
1
Jan 31, 2011
#1
I come from a background in mycology. Since college I've "helped" some friends acquire various strains of fungus that aren't always considered edible. For the past few years I've been holding on to a good collection of laboratory materials that have been sitting in storage. I love doing lab work, but horticulture rarely calls for it. So, I decided to find a happy medium.

Over the past 8 months I've been researching the viability of undergoing the mass production of clones via plant tissue culture.

Not only is PTC a viable way to produce large amounts of clones with the smallest amount of genetic material, it is produced with minimal amounts of energy, nutrients, and risk. The only variable that becomes trivial is time. PTC takes months instead of weeks to produce viable clones for production purposes. However, given an ample amount of investment (time) a technician could produce thousands of clones that can be stored in a fraction of the space of a mother plant for an indefinite amount of time.

Imagine storing the genetics of 50 different mothers in a single college-sized dorm fridge. Or even better, imagine raising 1000 clones for an entire warehouse underneath a single two-bulb fluorescent fixture. It's possible.

To date there is little research available on using PTC to cultivate cannabis. The studies that ARE done, are more focused on applications involved in activating genes in non-THC producing plants, or "supercharging" the THC producing mechanisms in the genetics of cannabis to create more potent genetics.

I have found a few good articles online. Both give good outlines for using different hormones as probable candidates to either educing rooting or the formation of callus. For those of you interested...

http://www.scribd.com/doc/14571756/The-Biotechnology-of-Cannabis-Sativa



They are great reads.

When I get some down time, I'll document on here some of my progress.
 
Quote Reply

Green81

Supporter
Premium Member
Posts
777
Reactions
8
Joined
Jul 28, 2008
Points
38
Jan 31, 2011
#2
look forward to your work, lots of pics I Hope.
 
Quote Reply

Cultiv8r

Posts
25
Joined
Aug 20, 2010
Points
1
Jan 31, 2011
#3
Does anyone have any first-hand experience on using IAA or IBA as a shoot-inducing hormone?
 
Quote Reply
O

organiclover

Posts
79
Reactions
252
Joined
May 6, 2010
Points
53
Feb 1, 2011
#4
only in science class in college
i remember one induces roots and the other induces shoots?
 
Quote Reply
O

organiclover

Posts
79
Reactions
252
Joined
May 6, 2010
Points
53
Feb 1, 2011
#5
great paper you posted BTW
i am very interested in this topic
not so much for biotech but mostly for tissue culture
 
Quote Reply
K

kr0n1x

Posts
21
Reactions
1
Joined
Feb 23, 2011
May 3, 2011
#6
Very interesting stuff. Any updates? I, too, dabble in myco, and I had never thought to X the two fields.
 
Quote Reply
J

jokalyx

Posts
13
Joined
May 3, 2011
Points
1
May 4, 2011
#7
kr0n1x, did you mean cross tissue culture and mycology? Because I am too interested in mychorrizae and mycology and have a small knowledge. But I know nothing about tissue culture and cell reproduction/duplication. Great thread, thumbs up Cultiv8r. Will follow this
 
Quote Reply
W

western5

Posts
5
Reactions
1
Joined
Oct 8, 2013
Points
1
Oct 8, 2013
#8
marijuana from test tubes is now available on the kindle through amazon
 
Quote Reply
Page 1 of 1 · Replies 1–8 of 8
1

Thread info

Replies 7
Views 4,823
Started Jan 31, 2011
Latest post Oct 8, 2013
Starter Cultiv8r
Forum Advanced Techniques & Problems

Latest posts

  • 2026 Outdoor Grows! let's see em!
    • Latest: Oldchucky
    • 31 minutes ago
    General Outdoor Growing
  • Tumble stumbles upon a Spider Farmer clip on fan.. Growing Blue Cheese, North Thunderfuck, Cali Blues, and Bruce Banner #2
    • Latest: Tumbleweed375
    • Today at 12:11 AM
    Grow Diaries
  • itscheese`s cheese thread
    • Latest: Itscheese94
    • Yesterday at 11:30 PM
    Introduce Yourself
  • Eternal Sun 2026 outdoor garden grow
    • Latest: EternalSun
    • Yesterday at 11:28 PM
    General Outdoor Growing
  • Big Al’s 2025 season indoor/outdoor grow in misery
    • Latest: EternalSun
    • Yesterday at 11:25 PM
    Grow Diaries
  • Home
  • Forums
  • Medical Cannabis Cultivation
  • Advanced Techniques & Problems
  • PTC - Micropropagation of Cannabis
  • Contact us
  • Terms and rules
  • Privacy policy
  • Help
  • Home
Community platform by XenForo® © 2010-2026 XenForo Ltd.
Menu
Log in

Sign up

  • Home
  • News
  • Classifieds
  • Forums
    • What's new Featured content New posts New Articles New articles New products Latest activity
  • Social
  • Strains
  • Live
  • Learn
  • Brands
X

Privacy & Transparency

We use cookies and similar technologies for the following purposes:

  • Personalized ads and content
  • Content measurement and audience insights

Do you accept cookies and these technologies?

X

Privacy & Transparency

We use cookies and similar technologies for the following purposes:

  • Personalized ads and content
  • Content measurement and audience insights

Do you accept cookies and these technologies?