What is there to test? What are the theories? Most plant biology is established science.
As I mentioned, testing theories or ideas to answer questions about the amount of light and dark during cloning and how it affects the plants. Questions like yours:
Regarding cloning, it might be interesting to consider the best way to stimulate the creation of new roots by manipulating light and dark duration. For example, would a complete lack of darkness inhibit or stimulate root growth?
Your own example proves that there are things to explore here and not everything is established, especially with cannabis because it’s been such a no-no for so long around the world. That means a lot of bro science has been passed around as fact. There was a time when many people growing thought birth control pills were the best thing for their plants.
This is further evidenced today by all the controversial things we do such as defoliating, there’s a huge amount of people for it and a huge amount of people against it, but where is the science?
As far as clones, most people said trimming the leaves will stimulate the root growth and remove focus from blah blah blah blah. Turns out, the most critical study I’ve heard showed as much as a 30% decrease in rooting when leaves are trimmed. Try to tell that to someone who insists on trimming the leaves every time. I trim my leaves when I have to but only for space, not to stimulate the roots.
Another example I can give is to do with bubble cloners. There are many people who insist you must use a cloning gel or powder and keep the water over 75°F. I used no gels or powders and my water temperature is closer to 70°F.
As far as lights out, the plant isn’t just constantly feeding on stored sugars in the roots, it gives back to the soil in an exchange of nutrients and other chemicals that only happens at night.
I have no idea what that means for a cutting trying to throw roots, but it suggests that plants would greatly benefit from a dark cycle as that’s what they’ve adapted to since the first plants evolved so many millions (billions?) of years ago.
I’m not pretending to be a scientist (obviously!

and I’m fully aware that observing a couple rounds of clones at home is not true scientific testing in a controlled environment.
I’m also not pretending to know everything about cannabis, as this thread demonstrates my learning curve with my first time bubble cloning.
I’m not trying to be a dick either, it just seemed like your comments were suggesting that all questions have been answered and we know everything there is to know about cloning, when none of us really do.
I suppose all I can hope to do is find what works best for my plants in my room. Maybe one day soon scientists will test all these theories under controlled conditions and give us proper and accurate information so we can all make the most of our plants.
