Graywolf
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Judging by the links you assume that the water dissolves or weakens the attachment of grains of chlorophyll within the chloroplast?
Unfortunately, these articles are not readily available to my understanding. My familiarity with the anatomy of the cell is limited only by -
I was interested in the location of the chlorophyll in the cell when suddenly got an emerald-green butane extract, washed from dry sepals peeled from fem seeds.
Green puzzled me, because the chlorophyll does not dissolve in nonpolar butane.
Then I decided that this strange behavior of chlorophyll depends on how the grains of chlorophyll are attached to the tissues of plants.
If they are held by some grease or wax, which are soluble in butane, after the dissolution of these compounds are released and the chlorophyll grains fill butane solution in a suspended state, but not dissolved.
With the naked eye, we cannot distinguish individual objects smaller than 50 microns and visually perceive suspension as a solution. Chl grains are 2-10 microns.
But even in these articles they write that the membranes are hydrophobic and water-soluble proteins are rare.
All chlorophyll (Chl)-binding proteins involved in photosynthesis of higher plants are hydrophobic membrane proteins integrated into the thylakoids. However, a different category of Chl-binding proteins, the so-called water-soluble Chl proteins (WSCPs), was found in members of the Brassicaceae, Polygonaceae, Chenopodiaceae, and Amaranthaceae families.
I used this polar->nonpolar gateway, transferring the desired resin from the green solution in ethanol to non-polar petroleum ether, it was the British lighter fluid "Newport."
The procedure was carried out in several stages.
I added small portions of petroleum ether, shaking vigorously, waiting for the separation of layers, took a yellowed layer of ether with a syringe and added a fresh portion instead of removed.
Of course, each successive portion was painted paler than the previous one.
Only in the last step I added water. Without water, the layers are separated much faster and a very inconvenient boundary layer of emulsion does not form.
Chlorophyll, as it is insoluble in a nonpolar solvent, always remains in alcohol and after adding water, chlorophyll remains in alcohol, diluted with water, but not in water.
In my opinion, this ancient technology from the era before the butane in no way indicates the solubility of chlorophyll in the water.
Unfortunately Water cannot wash away Chlorophyll.
Judging by the links you assume that the water dissolves or weakens the attachment of grains of chlorophyll within the chloroplast?
I was tossing Water Soluble Chlorophyll Binding Protein on the table as the possible elusive water soluble substance binding the chlorophyll in the plants and allowing water washing to exhibit some of the properties it does.
They are proteins that are soluble in water, have some solubility in alcohol, and are insoluble in non polar solvents.
Broken loose, the chlorophyll could form micelle, by clumping with their polar ends out and their hydrophobic tails in, which would allow them to be transported by the water.
Freezing would make them less available to the water, and with them in place, the chlorophyll would be protected from the alcohol.
What that doesn't explain, is the light green hue BHO picks up without the presence of alcohol.
That brings us to the definition of insoluble, which gets lightly tossed about as an absolute, when in fact it is a relative term. The alkane butane, considered insoluble in water, is not totally and will hold up to 32ml/L water.
Since there are no simple oxygenated alkane alcohols present, what is responsible for extracting the green?
I submit that polar chlorophyll is also slightly miscible with polar water, because it has both polar hydrophilic surfaces and hydrophobic surfaces.
Unfortunately, these articles are not readily available to my understanding. My familiarity with the anatomy of the cell is limited only by -
I was interested in the location of the chlorophyll in the cell when suddenly got an emerald-green butane extract, washed from dry sepals peeled from fem seeds.
Green puzzled me, because the chlorophyll does not dissolve in nonpolar butane.
Then I decided that this strange behavior of chlorophyll depends on how the grains of chlorophyll are attached to the tissues of plants.
If they are held by some grease or wax, which are soluble in butane, after the dissolution of these compounds are released and the chlorophyll grains fill butane solution in a suspended state, but not dissolved.
With the naked eye, we cannot distinguish individual objects smaller than 50 microns and visually perceive suspension as a solution. Chl grains are 2-10 microns.
But even in these articles they write that the membranes are hydrophobic and water-soluble proteins are rare.
All chlorophyll (Chl)-binding proteins involved in photosynthesis of higher plants are hydrophobic membrane proteins integrated into the thylakoids. However, a different category of Chl-binding proteins, the so-called water-soluble Chl proteins (WSCPs), was found in members of the Brassicaceae, Polygonaceae, Chenopodiaceae, and Amaranthaceae families.
I used this polar->nonpolar gateway, transferring the desired resin from the green solution in ethanol to non-polar petroleum ether, it was the British lighter fluid "Newport."
The procedure was carried out in several stages.
I added small portions of petroleum ether, shaking vigorously, waiting for the separation of layers, took a yellowed layer of ether with a syringe and added a fresh portion instead of removed.
Of course, each successive portion was painted paler than the previous one.
Only in the last step I added water. Without water, the layers are separated much faster and a very inconvenient boundary layer of emulsion does not form.
Have you tried using saturated brine, instead of plain water?
Chlorophyll, as it is insoluble in a nonpolar solvent, always remains in alcohol and after adding water, chlorophyll remains in alcohol, diluted with water, but not in water.
In my opinion, this ancient technology from the era before the butane in no way indicates the solubility of chlorophyll in the water.
Unfortunately Water cannot wash away Chlorophyll.
Plain water also washes green out of plant material in our bubble washing machine, so you can see why I'm not just jumping on the total insolubility/miscibility thang?
The bubble machine water looks like a colloidal suspension, but it is still being washed away by the water. How else did it get there, even as a collodial suspension, from its position locked in the plant material?
Do you have an alternate theory leaving chlorophyll totally insoluble, and yet explaining the observable?
Interesting read, thanks Joe and Graywolf!
Starting off with 99% iso, during the purifying steps (brine wash etc) is there a significant advantage of using hexane instead of acetone?
look at this weird shit i made from acetone...is it safe to smoke?? i wouldnt think sooo_OYes. Acetone isn't non polar.
look at this weird shit i made from acetone...is it safe to smoke?? i wouldnt think sooo_O
It smells SOOO GOOD though! its trippy.
i think im gunna try and wash it with 99% and then evap that...i cant find hexane for shit!View attachment 230238
you can smoke it. i would vac purge it for a bit first. well for a while, but you can smoke it. im sure its the bomb
what method did you use with the acetone?
ok here's a question, since Methanol and Hexane are not miscible, if you simply washed hexane with methanol, would that separate the chlorophyll/cannabinoids as well?
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