Creating clones from roots no cutting

[Mrmitten]

I've found a little bit of info and discussion by searching Google but not much info on this topic. Has anyone heard of creating clones from roots and does anyone have a guide they can point me too. I had a very nice Chemdog clone that I recently cut in dwc. I didn't take any clones plus I don't really plan on starting my tent back up until fall (no central air and summer electric rates are too high plus I have a nice harvest). Reading they take quite sometime to take hold it clicked this might be a nice experiment for me to do as I still have the bucket going from the clone question (has a single leaf some of the stem and root ball, originally i planned on trying to reveg this then take clones from that as I have time and it would require limited power). Thoughts anyone ever heard of this technique or is it just a myth?

 

[mysticepipedon]

I never heard of it, but there's a lot of stuff I never heard of. I wonder if this is about leaving a bit of stalk that can sprout a branch?

 

[lvstealth]

not sure how that would work. the root is the root because it is told to be a root, unless it has some bit that tells it to make a leaf, and a place (a node on a stem) to make that leaf, i dont see it being able to

if you plant a seed, it pops, and you pluck the shell left on and OOPS, take off the whole top. it will live, and get taller, but no node and it wont put a leaf out on that stick. (yup, THAT happened) the stick is still living, but i have finally given up (happened 1st of mar). it has a decent rootball, and a stick. but cant see it ever making green

 

[lvstealth]

you might be able to reveg her. you say it has a leaf? can you post a picture?

ive had some fun with the revegged "monsters". they look and act crazy and monsterish!

if you have the time, you might get the harvested plant to reveg (maybe... id need to see where and how the leaf looks.) but im not grasping the concept of a root turning into a plant.

there are plants you can do that with, most things that propagate with runners - like spider plants or hydrangea, can grow from roots. but that is in the programming of the plant. cannabis doesnt have runners.

some root plants can grow a new plant when only roots are left, but in actuality it is the veggie growing it with its own new roots, just comes from the root with nothing up top.

if they had a complete genetic sequence for cannabis, those questions would be answered in the absolute.

 

[Anthem]

I've found a little bit of info and discussion by searching Google but not much info on this topic. Has anyone heard of creating clones from roots and does anyone have a guide they can point me too. I had a very nice Chemdog clone that I recently cut in dwc. I didn't take any clones plus I don't really plan on starting my tent back up until fall (no central air and summer electric rates are too high plus I have a nice harvest). Reading they take quite sometime to take hold it clicked this might be a nice experiment for me to do as I still have the bucket going from the clone question (has a single leaf some of the stem and root ball, originally i planned on trying to reveg this then take clones from that as I have time and it would require limited power). Thoughts anyone ever heard of this technique or is it just a myth?

I am not complete sure that you can do this with Tissue Culture, regardless if you can or cannot. The need for Sterile environment is far above what a hobby grower can achieve.

 

[Mrmitten]

Here is a pic of whats left of it. I still have 1 other in the tent that hasn't quite finished up (fairly sure its a mislabeled sativa from seedbank). Its been hanging in for the last 2 weeks (a few flower nodes even popped up (or were already there and smaller) I've been picking them off as I heard it isn't good for reveg). I still can't switch to 24 hour light quite yet as I don't think this other plant is done. I'm in no rush to fill the tent up and have another run so I'm totally up to experiment with something for fun.

 

[Mrmitten]

 

[LexLuthor]

It's a lot more efficient to just take clones. If you didn't take clones then pop new seeds. I don't see any benefit of wasting electricity and time on a plant that has already been harvested. If you think you missed out on a 'keeper', you're probably wrong, especially considering you only have 2 plants.

 

[Moe.Red]

Yes, it can be done. Anthem is right, unless you have invested in the lab and techniques to do it, it is too late for you at this point.

It's not just the thousands you will spend to get sterile (my wallet has a hole in it where money used to be) it is the experience you need to be successful. Most tissue culture uses the apical meristem cells for good reason. I would not want my first try to be with a fully differentiated root cell that is FAR from sterile to begin with. The apical meristem is used because it is not yet connected to the plant's circulatory system - clearly every root cell is. Tough job for a beginner.

I also think Lex is right - probably not worth the effort even if you were lab ready - there are lots of other plants out there to experience - go pop some seeds man!

 

[Ponky]

Root cloning is for certain house plants and stuff. Not marijuana. Tissue culture is the step used when the material is limited.

 

[Moe.Red]

Tissue culture is the step used when the material is limited.

huh?

 

[Ponky]

huh?

It's a method of propagation using Petrie dishes and agar agar and proprietary ingredients.

Complete Guide to Cannabis Tissue Culture 101 - Cannabis Tech

Cannabis tissue culture cultivation represents a transformative approach to propagation in the cannabis industry. The science of tissue culture (TC) was

www.cannabistech.com

There is a way to use this technique to make hybrids. Without ever using seeds.

 

[Anthem]

Yes, it can be done. Anthem is right, unless you have invested in the lab and techniques to do it, it is too late for you at this point.

It's not just the thousands you will spend to get sterile (my wallet has a hole in it where money used to be) it is the experience you need to be successful. Most tissue culture uses the apical meristem cells for good reason. I would not want my first try to be with a fully differentiated root cell that is FAR from sterile to begin with. The apical meristem is used because it is not yet connected to the plant's circulatory system - clearly every root cell is. Tough job for a beginner.

I also think Lex is right - probably not worth the effort even if you were lab ready - there are lots of other plants out there to experience - go pop some seeds man!

Moe.Red I have seen this done before. When I have seen it done they used the stem of the plant and they did clean the material in ISO before going under the hood. Next the ends of the stem were cut. I am assuming this is to remove the dead cells from the ISO. Are the Apical Meristen cells inside the stem

 

[Anthem]

It's a method of propagation using Petrie dishes and agar agar and proprietary ingredients.

Complete Guide to Cannabis Tissue Culture 101 - Cannabis Tech

Cannabis tissue culture cultivation represents a transformative approach to propagation in the cannabis industry. The science of tissue culture (TC) was

www.cannabistech.com

There is a way to use this technique to make hybrids. Without ever using seeds.

Just because there is a way, it does not mean it is cost effective for the hobby grower.

 

[Moe.Red]

It's a method of propagation using Petrie dishes and agar agar and proprietary ingredients.

Complete Guide to Cannabis Tissue Culture 101 - Cannabis Tech

Cannabis tissue culture cultivation represents a transformative approach to propagation in the cannabis industry. The science of tissue culture (TC) was

www.cannabistech.com

There is a way to use this technique to make hybrids. Without ever using seeds.

Yeah, I know what it is and I do it. I don't understand the "when material is limited" part.

 

[Ponky]

Yeah, I know what it is and I do it. I don't understand the "when material is limited" part.

If you had a plant that was just a clump of roots with maybe some stalk and a few specks of green. You might be able to culture the plant. To make a mother plant. Where as that's too small to clone. Like if you grew the best bag seed ever and were desperate to save the genetics that would be the only way.

 

[Moe.Red]

Moe.Red I have seen this done before.

Me too, in my house.

Are the Apical Meristen cells inside the stem

Actually, I think that might be wrong. I have read they are at the tip of the top growth and tip of the roots in cannabis. You want to get away from the vascular structures.

 

[Moe.Red]

I guess this is all irrelevant. OP cannot do it at this point, and none of this is how or why I do it - it's like putting a square peg in a round hole.

I don't have the skills to make vibrant clones from a clump of left over root, and don't really plan on acquiring them. Not really the point.

 

[Anthem]

Me too, in my house.

Actually, I think that might be wrong. I have read they are at the tip of the top growth and tip of the roots in cannabis. You want to get away from the vascular structures.

That is what I have seen on videos, I am sure they used the just stem and not the tops or roots. I have no real world knowledge of how to do it, I have just watched the stuff on you tube.

 

[Anthem]

I guess this is all irrelevant. OP cannot do it at this point, and none of this is how or why I do it - it's like putting a square peg in a round hole.

I don't have the skills to make vibrant clones from a clump of left over root, and don't really plan on acquiring them. Not really the point.

be careful with that someone is going to post up one of those POS tissue culture kits that is available. Such a joke, the level of sterility required is not funny!