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You will also need a diffuser at the injection end to hope to touch the material on both sides of the injection orifice, where the inefficiency is greatest.
This is much easier if using straight liquid--you can slightly increase flow until the pressure applied is enough to force out the air from the top.
Here is a chromatography reservoir--the scientists solution to this (usually it will be attached to a flow rate controller as well as a valve closing both of these off from the column.