Your co2 should be ran during the the time that the lights are on only as this is the time the plants are photosynthesizing. The co2 should be kept at a rate of 1500 ppm (parts per million). Most flow regulators designed for horticultural use come with mathematical formulas to help you figure out where you need to be in comparison to the size of your grow space. The size of your room will dictate how long the 20lb tank will last. You could also use an atmospheric controller to monitor your room's co2 levels. I'd recommend running your room temperature at 80 degrees F if using co2. Dramatically taper the co2 down for the last second to last week of flowering to 600ppm. Then the last week of flower turn it off or let it run at 300ppm. Hope this helps mon! By the way, I'm about to try filling a 5x5 tent with the contents of a 20 lb tank to kill some root aphids. Has anyone tried this? Does it work? HELP! I got a 35 x 40 foot room to 5000 ppm for an hour with 2 -20 lb tanks. A friend and I were experimenting. You really need to be above 10,000 ppm to kill bugs, or anything for that matter so be careful if you're experimenting, but these damn things are in rockwool cubes so I don't know if its going to work or not. Root aphids are the worst.