SQUIG!
In this article http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf
it says in the abstract "MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip.
Whats 0.2 mg·L-1
Yes for your 1M solution calculation you were correct.
0. 2mg*L^-1 is a special mathy way to say mg/L or milligrams per liter.
Also, you mentioned the fizzing--next time, add the KOH more slowly--and never add water to the powder, always powder to water.
This is an exothermic reaction and it can be bad news bears if you rush it--slow and careful = not losing your eyesight.
ALWAYS WEAR EYE PROTECTION WHEN MIXING CHEMICALS--ALWAYS!!!!!!!!!!! The glasses should specify on them somewhere "z78" compliance.
So what is, or where are you getting Dropp? Do you purify it?
If I make a 1L stock solution of TDZ how long does it store for in the freezer?
Dropp is the commercial version of TDZ; it's used to defoliate cotton in the field. If you live in cotton country, it's all over the place. Several plant growth regulators are used commercially (Liberty, for example) in large quantities in a form that is vastly less expensive than the lab counterpart.
As for TDZ in the freezer- it should store indefinitely when frozen at -20C (your typical freezer).
>and for my other question about the .8% (w/v) when i was looking through plants from test
>tubes they have a ton of recipes for different plants a lot of them had 30 grams of sucrose and 8
>grams of agar per L so im assuming thats that.
These components are often used in these concentrations. Typical for sugar is somewhere between 20 and 35 g/L (depending upon many factors, including the crop- up to 50 g/L for potato microtubers, but more is not always better!), and 8 g/L agar depends upon several factors including the gel strength, concentration of nutrients, etc. Try some batches at 8 g/L of A111 PhytoTech agar and see how well it gels; too strong isn't necessarily bad- the roots will still penetrate- but it's wasteful and agar is expensive so when you're making hundreds of liters, it's common to tickle the dragon's tail and reduce the amount of agar to the point where the gel is very weak- but the plants are still well-supported physically in this fashion.
it's common to tickle the dragon's tail and reduce the amount of agar to the point where the gel is very weak- but the plants are still well-supported physically in this fashion.
agar is expensive so when you're making hundreds of liters, it's common to tickle the dragon's tail and reduce the amount of agar to the point where the gel is very weak- but the plants are still well-supported physically in this fashion.
I grow some ornimental plants and thought about doing this for some of the rare stuff. What would it cost to set up, just good enough to mess around with? I could build some stuff. Im just looking for a ballpark number, so i can figure out if its worth starting.
Lets talk culture vessels. My plan is to use my old half pint jars I have used for mycology work. I image they need gas exchange?
In PFTT they show liquid media and build little bridges to hold up the cuttings for some plants. Very cool!
OK I mixed up some media a while ago. I hadn't really thought it through and winged it. I messed up on my TDZ measurements. Anyways I ended up with a .25mg to ml ratio on 4 jars and a .45 mg to ml on 4 and then 8 jars with really strong amounts which i have not yet inoculated. And i added 2mg of Gibberellec acid to all of them.
0.25 mg/mL TDZ is way too high. Do you mean a final concentration of 0.25 mg per liter?
Thanks for the love yarra,
Out of curiousity what was the company you applied for? I have heard a lady on a video cinfidently say " you can turn one plant into a million in under ten months" which by no means I have that type of facility/dollars/reason to do numbers like that, but I believe it is possible no doubt.
I have heard of in vitro breeding, but currently am not trying to accomplish or even think about (though as soon as i heard it i was like WAAAA!!!!) that untill I have the basics down to a science. I will definitley be taking things slow in this operation, experiment by experiment. I have a major issue with trying to jump forward too fast and try new things before I even know the basics. ( even my first grow many many years ago was a very complicated aeroponics system, which even now as a pretty experienced grower would have a hard time keeping up with) so i will be keeping the horse in front of the cart on this one. Meaning this thread should be around for a long time to come.
If there is any information you know of please share it with me, I am far from a scientist, but maybe one at heart.
much love,
rns
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