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In vitro micropropigation

  • Thread starter Thread starter rootsnshoots
  • Start date Start date Jun 12, 2012
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In vitro micropropigation

rootsnshoots Jun 12, 2012 106 Replies 22,450 Views
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oxanaca

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#101
western5 said:
Marijuana from test tubes is now available on Amazon. It is a book that has an illustrated step by step guide for TC of cannabis
Click to expand...

thanks so much. i read the preview they provide for free and it put some powerful ideas in my head. it mentions how he had found he was able to multiply autoflowering cannabis containing ruderalis genetics, and skip paying money for overpriced seeds with unpredictable results. it seems like the info is worth $25 im gonna purchase it just to see.

now im not a fan of autoflowering strains. but can you imagine starting out a bunch of autoflowering strains from seed. creating about 20 copies of each. growing them out, inside, to find the best strains. then multiply your best cultures to create an army of plants ready to go outside may 20th. by august first you could have an army of little plants that were flowered in the best sun of the year. and not really have to worry about choppers seeing your shit since their being harvested 2 months before the usual september 1st harvest and their so much smaller than conventional plants.
 
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oxanaca

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#102
haha i just bought it, ill provide an update in a few days and tell you people if it was worth the money
 
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oxanaca

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#103
alright the text was kind of lame only 56pages and sort of basic. i would only recommend to someone extremely interested, even the pictures werent that interesting low resolution. i think i could have written this book myself.

here are some 1 liter formulas outlined in the book

Conclusions: For the home hobbyist, the most reliable protocol is as follows:
Stage I from cuttings/ Stage II divisions:
1 MS with vitamins
1 ml PPM (not necessary with good aseptic technique)
.5 ml IBA at 1mg/ ml =1/2ppm
1 ml Kinetin at 1mg/ ml =1ppm
9 g/ l agar
30 g/ l sucrose (household sugar)

Stage III Rooting:
1 MS with vitamins
1 ml PPM (not necessary with good aseptic technique)
1 ml IBA at 1 mg/ ml =1ppm
1 ml TDZ at 0.1mg/ ml =0.1ppm
9 g/ l agar
30g/ l sucrose (household sugar)
0.5g/l activated charcoal (0.7/ l = ¼ teaspoon for home measurement purposes).
 
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oxanaca

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#104
im gonna try these when i get a shot. i would be interested to see anyone elses results as well.
 
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neverbreak

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#105
thanks for lettin us know about the book. i was thinkin about it, but still don't have the necessary equipment to start tinkerin with tc. someday...

neverbreak
 
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oxanaca

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#106
oxanaca said:
alright the text was kind of lame only 56pages and sort of basic. i would only recommend to someone extremely interested, even the pictures werent that interesting low resolution. i think i could have written this book myself.

here are some 1 liter formulas outlined in the book

Conclusions: For the home hobbyist, the most reliable protocol is as follows:
Stage I from cuttings/ Stage II divisions:
1 MS with vitamins
1 ml PPM (not necessary with good aseptic technique)
.5 ml IBA at 1mg/ ml =1/2ppm
1 ml Kinetin at 1mg/ ml =1ppm
9 g/ l agar
30 g/ l sucrose (household sugar)

Stage III Rooting:
1 MS with vitamins
1 ml PPM (not necessary with good aseptic technique)
1 ml IBA at 1 mg/ ml =1ppm
1 ml TDZ at 0.1mg/ ml =0.1ppm
9 g/ l agar
30g/ l sucrose (household sugar)
0.5g/l activated charcoal (0.7/ l = ¼ teaspoon for home measurement purposes).
Click to expand...

i feel kind of bad for saying " marijuana from test tubes" was lame. it was not lame, im just already adept at all the processes involved.

for someone who isnt proficient at growing mushrooms, hasn't yet honed their sterile technique, and has never mixed their own fertilizers. i believe this text will defintily be of great value, well beyond the $25 cost.
 
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entropy99

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#107
Any updates rootnshoots?
 
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Thread info

Replies 106
Views 22,450
Started Jun 12, 2012
Latest post Nov 20, 2013
Starter rootsnshoots
Forum Advanced Techniques & Problems

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