Microbial Innoculant Em*1 Created By Dr. Teruo Higa

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Samoan

Samoan

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Anyone who is using Effective Micro-Organisms to aid in growing there medicine.
I strongly suggest taking a look at Dr. Teruo Higa's web page and reading a little about him one of the pioneers of micro-biology and how he uses Effective Micro-organisms. I will be making
Activated EM 1 and adding it as a Microbial Innoculant to my aerated compost tea mixture of OG Bio war, ewc, alphalpha, Mendocino honey molasses, and sea weed kelp extract
 
Microbial innoculant em1  created by dr teruo higa
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Samoan

Samoan

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Enhance Organic Compost Tea with EM•1® Facultative Microbes
teabrewersanfran.jpg
Compost tea is a liquid extract made from aerobically bubbling air through compost in order to grow aerobic microbes. The composting tea’s quality is directly related to the quality of the compost and has a very short shelf life, roughly 12-24 hours. The aerobic microbes require air to stay alive. Once the air is gone, they start to die off. The quality of the tea is dependent on the quality of the compost and cleanliness of the containers. For this reason, organic compost tea quality varies from batch to batch as the microbial populations vary with each new batch.

EM•1® contains facultative microbes, is consistent in quality, and is very stable, with a shelf life of several years. Adding EM•1® facultative microbes to the compost process enhances the organic compost process and ensures consistently high quality compost tea.

The picture shown is a commercial-sized tea brewer used at Harding Golf Course in San Francisco. They brew compost tea and inoculate with EM•1® Microbial Inoculant as part of their greens program.

We recommend you make your composting tea with EM•1® Microbial Inoculant to ensure a high quality compost (see our composting section). Once the compost is complete, you can make a high quality compost tea. For helpful tips and guides to using EM Technology® see below or click on Solutions above.
 
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Samoan

Samoan

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Add AEM1 @ 1:50

I added 18Tsp to 5Gal of ACT.

I believe some farmers add ACT to there nutrient mixture, whether it be synthetic or organic @ 1:5

Activated EM 1, if sealed properly, will last 40-60 days.

SUPER CHARGE
 
P

PharmHand

846
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I use activated em1 too. I can't say for certain how much it helps or if it does at all.... Like with ANY supplement too many factors to say whether it's actually doing anything at all. But it seems legit, and I love the smell of it activated. I wanna take a drink of it every time I use it.... Love me some fermented juice mmmmmm kombucha:)
 
P

PharmHand

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It's super low ph tho, might conflict with the more ph neutral bacteria brewed in act tho no?
 
Samoan

Samoan

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It's super low ph tho, might conflict with the more ph neutral bacteria brewed in act tho no?
Honestly I'm not sure about the ph, but yes my act is 6.5-7.0
The aem1 is 3.8...

I hope someone can elaborate
 
Samoan

Samoan

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Your Buck: Make Your Own Activated EM•1® For Less Than $4 Per Gallon!
EM•1® microbial products can be grown one time for economical purposes. This "growing" or "brewing" process is called Activation (it used to be called "extension"). It does not mean the microbes in EM•1® are not active; they are. It is just a term EMRO developed years ago. Activation involves taking one part EM•1®, 1 part molasses, and 20 parts water. Numbers can be rounded up or down a bit according to the size of the container and are not crucial to the outcome of the final product.

Equipment

As with beer and wine, the colder the temperature, the longer the fermentation takes to complete. And, the process is done without air. Any local home brewing supplier will have the materials you will need to successfully activate. These items include airlocks, carboys, tubing, etc. For an example of what an airlock looks like and costs, click here.Airlocks and other brewing equipment can be found online through many home brewing suppliers or at a local home brew supply. If you want to keep it really simple, you may use a clean soda bottle with a screw on top. These bottles are very good because they can take a tremendous amount of pressure and can be found virtually anywhere.

  • Use a clean airtight plastic bottle or container or large tank with screw-on lid.
  • Use a container about the volume of the recipe being made. Avoid containers that have large air space as the excess air tends to interfere with fermentation and do not use containers with loose lids such as 5-gallon pails.
  • Be prepared to either make an airlock or manually release the pressure on a daily basis.**
  • Use only fresh, clean water. Tap water is fine (yes, even if chlorinated).
  • Use blackstrap molasses. Avoid using beet molasses or corn syrup as they tend to spoil easily.
Ingredients (examples of common sizes)

Ingredient
1 Quart
1 Gallon
5 Gallons
55 Gallons
275 Gallons
1200 Gallons
EM•1®
1.5 oz
3/4 Cup
32 oz
2.5 Gallons
12 Gallons
55 Gallons
Molasses
1.5 oz
3/4 Cup
32 oz
2.5 Gallons 12 Gallons 55 Gallons
Water (110ºF) 29 oz
116 oz
4.5 Gallons 50 Gallons 251 Gallons 1090 Gallons

Directions
  • Mix ingredients in the container. Check the initial pH with a pH meter or pH paper.
  • Put on an airtight lid (preferably a screw on lid),
  • Attach airlock
  • Ferment at room temperature for 3-5 days.
  • Some time between days 3 and 5, remove the lid and check the pH of the liquid using a pH meter or pH paper. If the pH is 3.8 or below, allow the fermentation to complete for an additional 5-7 days.
  • Toward the end of the fermentation, check the smell of the product. It should have some alcohol smell, some white flakes on it and look and smell similar to the original EM•1®. If all these are true, it is ready to use.
Checking pH
The initial fermentation process takes roughly 3-5 days. During this time sugars are being digested by the microbes. As they do this, they produce various acids that cause the pH to drop. pH is the measurement of Alkalinity (bases) and Acidity (acids). You can easily measure this using some pH paper. The pH paper we sell has a perfect range for testing this. It goes from 3.0-5.5. (Electronic pH meters are sold through scientific companies; however, they require maintenance and calibration and can vary in readings between meters. If you choose to use an electronic meter, maintain it well and use the same meter every time.) The pH paper we sell is very accurate and consistent. There is a color chart on the side of the package that is used to gauge the pH. You generally do not start checking the pH until about 3-5 days after you mix and start the fermentation. However, if you would like to know the starting point and the finishing point, you can check the mixture before fermentation.

When the pH of the solution drops to 3.8 or below and has rested at this pH for 5-7 days, the Activated EM•1 ® is ready to use. The target pH is 3.5.

You will have about 45-60 days to use it at this point.

**During the fermentation process, pressure builds up in the containers as the microbes digest the sugars. To avoid rupturing the container, "burp" it and reseal. Burping may have to be done one or more times per day. You may also use airlocks or blow off tubes similar to what is done in brewing wine or beer.

STORAGE



Activated EM•1® should be kept in an air-tight container to keep it anaerobic and used within 45-60 days after the pH drops below 3.8.

Store Activated EM•1® at room temperature, 68ºF to 86ºF (20ºC-30ºC) out of direct sunlight. Refrigeration is not necessary.

NOTES



Speed of fermentation: Usually larger batches ferment faster than smaller ones. Sometimes the pH drop will only take 24 hours. Usually we plan for the pH drop (below 3.8) to take about 3 days.

Temperatures and seasonal changes in fermentation times: EM•1® likes warm conditions. Keep the material in a relatively warm area while it is fermenting. (for example, a cellar is too cool for it to ferment quickly.) In the winter, when the temperature is low, place the fermenting mixture somewhere to keep it warm. There is no need to incubate or heat the product as this actually tends to prevent the pH from dropping.

Type of Molasses: Organic molasses tends to have some type of pH buffer in it, which sometimes prevents the fermentation process. You can add in an equal amount of vinegar (apple cider, wine, etc.) to the molasses to help break the pH buffer.

Air causes the Activated EM•1® to spoil. This includes extra air space in the bottle. Be sure to leave as little airspace in the bottle as possible when fermenting.

Try not to make more product than you can use within a month or two to avoid wasting product.

If Activated EM•1® has a foul smell or the pH rises above 4.0, the solution is likely contaminated with something and should be discarded. This material is fine to pour into a long-term compost pile or down a household drain.

What to do with your Activated EM•1®
Now that you have some sweet smelling Activated EM•1, you've let it mature for 7-10 days after it reached 3.5, you need to start using it. Simply replace any of the application rates on our website with the "Activated EM•1®" you made. That's it!
 
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Samoan

Samoan

260
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Making Activated EM (AEM)
Effective Microorganisms (EM) can be activated so that you end up with approximately 20 times the original amount. This is definitely not necessary, but it is often done to save money. It also helps to awaken dormant microbes. Application rates are the same either way.

Ingredients (per 1L):
1 part EM (40ml) -research shows that up to 1.5 parts may be better; I now use 1.25 parts (50ml)
1 part Organic Blackstrap Molasses (40ml)
20 parts dechlorinated water*, not distilled or reverse osmosis (800ml)
Clean, airtight bottle (1L), generally plastic with screw-on lid (not glass) – can use bigger jugs, too. (do not use a jug in which a bad batch has previously been fermented, unless it has been washed, scrubbed, bleached, and cleaned with EM, and then all of these steps repeated again)
pH paper is very useful to determine if your batch is good

Optional and optimal (I currently sell the last 3 in 1L bottles):
1/4 tsp ceramic powder to structure the water (more won't hurt)
1/2 Tbsp rock dust for minerals
1/2 tsp sea minerals for many good things
1/2 tsp liquid kelp for many good things
1/2 tsp liquid fish for many good things
There are several other ingredients not listed here that I use in my own activations.

*Dechlorinated water is not necessary, but definitely beneficial, as chlorine kills microorganisms. Tap water can be dechlorinated by letting it sit out for 24 hours. If the tap water has chloramine (such as in Victoria), a small amount of humic acids will help to tie it up (1/2 tsp per litre).

Directions:
1. Heat some of the water to 115-125F
2. Pour water into your bottle and dissolve molasses
3. Mix in any of the optional ingredients and then mix in the EM
4. Add the remaining water and stir and/or shake very well.
5. There should be an airspace equivalent to about 10% of the bottle on top
6. Tighten lid and keep warm (95-110F is optimal) for at least 2 weeks, preferably longer**
5. After the first few days, the container needs to be burped every day or two, as gases are formed that will expand the bottle. The mixture should be stirred and/or shaken daily for the first 3 weeks, too.
6. Can be used when pH is below 3.9, but preferably below 3.7. It should also have a sweet smell (it may smell a bit when you initially take off the cap, but if that persists for more than 10 minutes of the cap being off, or if the pH is 3.9 or over, it should not be used; note that if you add fish and/or kelp, it may have a smell to it, but that is okay).
7. Product is better left for at least 4 weeks for highest benefits
**Can be kept warm in an oven with the oven light on (put a note on the oven), in a cooler with a terrarium heater or some other heat source, or many other ways. The whole process can be done at 70F, but it will take 6-8 weeks, and the odds of failure are higher. Even keeping it warm for the first few days is helpful.

Storage:
1. EM Mother Culture stores for 6-24 months, or potentially a few years
2. Activated EM stores for a month or two, or potentially 2 or more years if it is a good batch. Cooler room temperatures will keep it longer (10-20C is optimal). Store it in the dark, with the lid tight.
3. While it is good to brew it with a bit of airspace and stir/shake it daily during the first 3 weeks of brewing, it is best to store it after that without any airspace and without any more shaking if you want to keep it stored for more than a month. The odds are, if you are brewing it for long term storage, you are probably brewing many bottles at once, therefore one of the bottles can be used to top off the rest, and then the remainder in that bottle can be used first.

Note:
Activating EM is not an exact science. As such, the occasional batch may fail to drop in pH to an appropriate level. You may try adding a bit more EM and other optional ingredients, or you may decide to throw it into the compost (or down the drain if it smells bad) and start again. Some of the
most common reasons for failure are:
1. Inappropriate containers (previously containing chemicals, putrefied food, bad AEM batches)
2. Poor water quality (distilled, reverse osmosis, polluted, very high in chlorine)
3. Filling the container too full during initial fermentation stages (best if only 90% full)
4. Not optimal temperature (too hot or too cold)
5. Not burping and stirring/shaking daily
6. Trying to cheat by using more molasses than EM

If you follow the directions above, you will be very likely to produce a beautiful activated EM.

Phil
 
P

PharmHand

846
143
Making Activated EM (AEM)
Effective Microorganisms (EM) can be activated so that you end up with approximately 20 times the original amount. This is definitely not necessary, but it is often done to save money. It also helps to awaken dormant microbes. Application rates are the same either way.

Ingredients (per 1L):
1 part EM (40ml) -research shows that up to 1.5 parts may be better; I now use 1.25 parts (50ml)
1 part Organic Blackstrap Molasses (40ml)
20 parts dechlorinated water*, not distilled or reverse osmosis (800ml)
Clean, airtight bottle (1L), generally plastic with screw-on lid (not glass) – can use bigger jugs, too. (do not use a jug in which a bad batch has previously been fermented, unless it has been washed, scrubbed, bleached, and cleaned with EM, and then all of these steps repeated again)
pH paper is very useful to determine if your batch is good

Optional and optimal (I currently sell the last 3 in 1L bottles):
1/4 tsp ceramic powder to structure the water (more won't hurt)
1/2 Tbsp rock dust for minerals
1/2 tsp sea minerals for many good things
1/2 tsp liquid kelp for many good things
1/2 tsp liquid fish for many good things
There are several other ingredients not listed here that I use in my own activations.

*Dechlorinated water is not necessary, but definitely beneficial, as chlorine kills microorganisms. Tap water can be dechlorinated by letting it sit out for 24 hours. If the tap water has chloramine (such as in Victoria), a small amount of humic acids will help to tie it up (1/2 tsp per litre).

Directions:
1. Heat some of the water to 115-125F
2. Pour water into your bottle and dissolve molasses
3. Mix in any of the optional ingredients and then mix in the EM
4. Add the remaining water and stir and/or shake very well.
5. There should be an airspace equivalent to about 10% of the bottle on top
6. Tighten lid and keep warm (95-110F is optimal) for at least 2 weeks, preferably longer**
5. After the first few days, the container needs to be burped every day or two, as gases are formed that will expand the bottle. The mixture should be stirred and/or shaken daily for the first 3 weeks, too.
6. Can be used when pH is below 3.9, but preferably below 3.7. It should also have a sweet smell (it may smell a bit when you initially take off the cap, but if that persists for more than 10 minutes of the cap being off, or if the pH is 3.9 or over, it should not be used; note that if you add fish and/or kelp, it may have a smell to it, but that is okay).
7. Product is better left for at least 4 weeks for highest benefits
**Can be kept warm in an oven with the oven light on (put a note on the oven), in a cooler with a terrarium heater or some other heat source, or many other ways. The whole process can be done at 70F, but it will take 6-8 weeks, and the odds of failure are higher. Even keeping it warm for the first few days is helpful.

Storage:
1. EM Mother Culture stores for 6-24 months, or potentially a few years
2. Activated EM stores for a month or two, or potentially 2 or more years if it is a good batch. Cooler room temperatures will keep it longer (10-20C is optimal). Store it in the dark, with the lid tight.
3. While it is good to brew it with a bit of airspace and stir/shake it daily during the first 3 weeks of brewing, it is best to store it after that without any airspace and without any more shaking if you want to keep it stored for more than a month. The odds are, if you are brewing it for long term storage, you are probably brewing many bottles at once, therefore one of the bottles can be used to top off the rest, and then the remainder in that bottle can be used first.

Note:
Activating EM is not an exact science. As such, the occasional batch may fail to drop in pH to an appropriate level. You may try adding a bit more EM and other optional ingredients, or you may decide to throw it into the compost (or down the drain if it smells bad) and start again. Some of the
most common reasons for failure are:
1. Inappropriate containers (previously containing chemicals, putrefied food, bad AEM batches)
2. Poor water quality (distilled, reverse osmosis, polluted, very high in chlorine)
3. Filling the container too full during initial fermentation stages (best if only 90% full)
4. Not optimal temperature (too hot or too cold)
5. Not burping and stirring/shaking daily
6. Trying to cheat by using more molasses than EM

If you follow the directions above, you will be very likely to produce a beautiful activated EM.

Phil
That's the procedure I use too. Here's the vid
 
Samoan

Samoan

260
43
LAB – Lactobacillus Serum Click here for a guide to culturing LAB

EM-1 – Effective Microorganism. First defined by Teruo Higa in the paper: The Concept and Theories of Effective Microorganism

Effective Microorganisms, aka EM Technology, is a trademarked term now commonly used to describe a proprietary blend of 3 or more types of predominantly anaerobic organisms that was originally marketed as EM-1™ Microbial Inoculant but is now marketed by a plethora of companies under various names, each with their own proprietary blend. “EM™ Technology” uses a laboratory cultured mixture of microorganisms consisting mainly of lactic acid bacteria, purple bacteria, and yeast which co-exist for the benefit of whichever environment they are introduced, as has been claimed by the various em-like culture purveyors. It is reported to include:
Lactic acid bacteria: Lactobacillus plantarum; L. casei; Streptococcus Lactis.
Photosynthetic bacteria: Rhodopseudomonas palustris; Rhodobacter sphaeroides.
Yeast: Saccharomyces cerevisiae; Candida utilis (These species are no longer cultured for, but some some palustris does end up from the basic LAB process).
Actinomycetes (no longer used in the formulas): Streptomyces albus; S. griseus.

So these days, for one reason or another, what is sold as EM-1 is Lactobacillus plantarum, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus delbrueckii, Bacillus subtilis, Saccharomyces cerevisiae, Rhodopseudomonas palustris.

“Effective microorganisms make amino acids useful to plants, and organic acids, polysaccharides and vitamins strengthen their immune systems. EM1 consists of a water solution that contains compounds that promote nitrogen fixation and photosynthesis, along with lactic bacteria, yeast and other components that these microorganisms need to live ” – (Shablin, 2006)

Commonly, you will be referred here when you learn about EM-1. Which you will notice is the same recipe when we speak of LAB. This recipe gives you LAB, not EM-1, as there are no PNSB / Yeast.
What is sold here is different then when we make LAB.
 
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