Aqua’s Hydro systems meet SPIDERFARMER SE 7000

[Aqua Man]

What I meant by reset was it would create a version that has the vibrance of a new seedling. This may be bro science but I was told HLV can make the jump in tissue culture as well. I have one plant that I receive that was a clone of a Tissue cultured mother. It did display the traits of cloning a new seedling.
I just wish I had a nice laminar flow hood like many of the Commercial mushroom guys use to preform tissue culture work!!!!

Easy to build bro

 

[Anthem]

Easy to build bro

I will look into it. I just wish someone on here that has built one would give me some pointers

 

[ImpulsiveGrower]

So guess who turned on the water today then went inside to make a call that lasted 2 hrs while completely forgetting he turned the water on… yeah i know guy is an idiot…

Hey it’s ok I’ve flooded my bathroom and basement like 5 times this summer. The float valve didn’t work worth a shit so I had to just time it out. Except I would forget to actually set any timer and then wake up hearing noises which was water dripping thru my bathroom floor into the basement. Luckily on the unfinished side that has a drain but still!

 

[Aqua Man]

I will look into it. I just wish someone on here that has built one would give me some pointers

What ya wanna know?

just watching a movie can help ya through DM after. The main expense is the filter

 

[Aqua Man]

Hey it’s ok I’ve flooded my bathroom and basement like 5 times this summer. The float valve didn’t work worth a shit so I had to just time it out. Except I would forget to actually set any timer and then wake up hearing noises which was water dripping thru my bathroom floor into the basement. Luckily on the unfinished side that has a drain but still!

Ouch man… kinda makes me feel fortunate

 

[Moe.Red]

I will look into it. I just wish someone on here that has built one would give me some pointers

I can help you

 

[Moe.Red]

Answer prob in here but haven’t read it yet.

Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable ...

www.ncbi.nlm.nih.gov

I started reading but after 30 minutes could not find what I was looking for. I'll spend some time on this over the weekend and see if I can find some useable results to start with.

Not so sure about using CRISPR myself, we will see.

 

[Moe.Red]

What I meant by reset was it would create a version that has the vibrance of a new seedling. This may be bro science but I was told HLV can make the jump in tissue culture as well. I have one plant that I receive that was a clone of a Tissue cultured mother. It did display the traits of cloning a new seedling.
I just wish I had a nice laminar flow hood like many of the Commercial mushroom guys use to preform tissue culture work!!!!

Personally I have never seen HLV.

If done properly tho, I think that virus elimination is one of the benefits of TC. If you take just the Apical Meristem, chances are the virus isn't yet there in an otherwise effected plant, and then you do a sterilization process with chlorine on the plant cells.

But again, no first hand experience.

 

[beluga]

You guys are bad for my wallet.

Just throwing that out there.

 

[Aqua Man]

I can help you

View attachment 1294851

View attachment 1294852

Very very nice setup

 

[Anthem]

Personally I have never seen HLV.

If done properly tho, I think that virus elimination is one of the benefits of TC. If you take just the Apical Meristem, chances are the virus isn't yet there in an otherwise effected plant, and then you do a sterilization process with chlorine on the plant cells.

But again, no first hand

Moe you are making look up the fancy words. But I learned something. Apical Meristem is in the new grow tips and roots. Responsible for cell division so that makes sense. The viroid may not have traveled to the new growth yet. That makes sense because the bro science way of trying to chase the biroid out of the plant is rapid growth and cloning.

 

[Aqua Man]

Moe you are making look up the fancy words. But I learned something. Apical Meristem is in the new grow tips and roots. Responsible for cell division so that makes sense. The viroid may not have traveled to the new growth yet. That makes sense because the bro science way of trying to chase the biroid out of the plant is rapid growth and cloning.

Thats exa how I understand it

 

[Moe.Red]

You guys are bad for my wallet.

Just throwing that out there.

Ohhh yeah, what are we buying???

 

[Anthem]

I can help you

View attachment 1294851

View attachment 1294852

Please correct me if I am wrong. Wooden box house the hepa filters. With prefilters around the intake to help keep the Hepa Filters clean? What CFM fan?

 

[beluga]

Ohhh yeah, what are we buying???

All this flowhood talk... I've been out of the fungiculture work for a little while now, but man if I didn't spend a lot of time building shopping lists for hoods I never ended up building. Now it's like 3x the price . Dayton blowers are like $2-300 now
Guess I'll whip out the old SAB in the meantime...

 

[Moe.Red]

Very very nice setup

It is about $1000US for everything there including the stainless sheet for the top.

Could go with 1 filter instead of 2, but honestly I wish I had 3 when I have a bunch of mushrooms to process. My bubba's barrel (pasteurizer) holds 200# of substrate per load. It does not all fit up there without gymnastics.

For a small lab, you could get away with a much smaller setup.

 

[Moe.Red]

Please correct me if I am wrong. Wooden box house the hepa filters. With prefilters around the intake to help keep the Hepa Filters clean? What CFM fan?

Correct. Lemme find the blowers

2 of these

Dayton 1TDT8 Blower, 797/549 CFM, 115V, 3.30/2.20 Amp: Electric Fan Motors: Amazon.com: Industrial & Scientific

Dayton 1TDT8 Blower, 797/549 CFM, 115V, 3.30/2.20 Amp: Electric Fan Motors: Amazon.com: Industrial & Scientific

www.amazon.com

2 of these

Amazon.com: Novatek F4010 HEPA Filter Novair 2000 24" x 24" x 11.5" : Tools & Home Improvement

Buy Novatek F4010 HEPA Filter Novair 2000 24" x 24" x 11.5": Furnace Filters - Amazon.com ✓ FREE DELIVERY possible on eligible purchases

www.amazon.com

The rest is just plywood and screws, and yes there is a prefilter made of just furnace filters to keep any dust out of the Hepa filters.

 

[Anthem]

It is about $1000US for everything there including the stainless sheet for the top.

Could go with 1 filter instead of 2, but honestly I wish I had 3 when I have a bunch of mushrooms to process. My bubba's barrel (pasteurizer) holds 200# of substrate per load. It does not all fit up there without gymnastics.

For a small lab, you could get away with a much smaller setup.

What are you using to sterilize? Atmosphere or pressurization

 

[Aqua Man]

What are you using to sterilize? Atmosphere or pressurization

You work infront of the hepa so the jars are only exposed to cleaned air. Generally let it run to clean the room air for a period of time is good practice. I built a positive pressure box for $100 because its more economical for a small setup. But a laminar flow hood is the Cadillac

 

[Moe.Red]

What are you using to sterilize? Atmosphere or pressurization

This is for the bulk substrate

85 Gallon Mushroom Substrate Steamer | Bubba's Barrels

Based on a design by Myers Mushrooms, our substrate steamer is the easiest, most cost-effective solution on the market. Not intended for use with grain spawn. A typical cycle will take 14-24 hours. Time is dependent on ambient temperature, substrate temperature, and heater wattage. The 85...

www.bubbasbarrels.com

And I use a pressure autoclave for the grain spawn bags

Amazon.com

Really hard to find autoclaves in stock since 2020 for --- reasons.