Aqua Man
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No hijack, all is good for discussion here man. Good for those looking to explore mycologyI grow those in tall wide mouth mason jars. They are friggin delicious.
Aqua, sorry about the super hijack here, got started and couldn't help myself
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And to bring it back to cannabis, this is what it looks like when you use great white from the very beginningNo hijack, all is good for discussion here man. Good for those looking to explore mycology
We’re you using RTV on dish for spore transfers?Probably my favourite part of mushrooms is the agar work and isolating mycelium… idk why but i could do that everyday for the rest of my life… mycelium are soooo fucking fascinating. I had some pan cyan spores i was isolating and had a isolate that would literally eat trichoderma for breakfast but the fucker wouldnt fruit for shit. Worked on it for months. Cloning is fun too. I kinda miss the tinkering with it but will be to busy to take that up again
I made my own jars and liquid cultures… for that i used RTV for the syringe plugs that i put into the holes i drilled in the lid. I drilled 2 holes in the lids one for syringe and the other for air exchange… some use cotton but i used a syringe cap that allows gasses but not moisture through and sealed then into the lids with RTV. I can find pics of them if you likeWe’re you using RTV on dish for spore transfers?
I am good. I can figure it out from thereI made my own jars and liquid cultures… for that i used RTV for the syringe plugs that i put into the holes i drilled in the lid. I drilled 2 holes in the lids one for syringe and the other for air exchange… some use cotton but i used a syringe cap that allows gasses but not moisture through and sealed then into the lids with RTV. I can find pics of them if you like
Yeah with liquid culture you dont even need a flow hood ime. Thats why i started doing that.With a hood you will not need RTV or ports.
You can start liquid cultures with water and dextrose (sterilized) and just add the spores in front of the hood. I also drop a magnet stir rod in there and pop the jars on a stir plate from time to time.
Agar plates work really well too, but are more expensive and not as quick to use when doing bulk stuff.
With the dextrose / water in a mason jar thing, you can keep it going indefinitely like sourdough starter.
Most of the recent talk in about Mushroom growing and not tissue culture. Tissue cultures needs similar sterilization but also requires additional prep for the tissue being cultured. It is probably the ultimate in regeneration of an old and tired mother plantFascinating discussion guys. @ImpulsiveGrower, you may want to check this tissue culture thing out bro!
Absolutely H2O hombre. H2 hombre? H2Ombre?Yeah with liquid culture you dont even need a flow hood ime. Thats why i started doing that.
i like the agar because you can easily distinguish the different sub species. If cloning though or working with an isolate definitely liquid culture gives you more to work with faster… i always use a stir bar in mine also
feel fre bro honestly… that’s exactly what i ise for my lidsI promise last hijack and I will shut up about mycology on your thread.
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I think they loving the o2… those babies are nicest roots i have seen. As the golden arches would say…. da da da da da…. I’m lovin it.So back to our local superhero and his brain that won't shut off, this ain't too bad:
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You think those roots are not loving the O2? These some really healthy plants my dude. I've never had ballistic growth like this before.
No way I get here without your - you wanna tell them the name?I think they loving the o2… those babies are nicest roots i have seen. As the golden arches would say…. da da da da da…. I’m lovin it.
absolutely incredible
Oxy-Gen OG for short… delayed but coming soon lol.No way I get here without your - you wanna tell them the name?
What can OG do for you?Oxy-Gen OG for short… delayed but coming soon lol.
Hands down the most rewarding part of it. Even in a still air box.Probably my favourite part of mushrooms is the agar work and isolating mycelium… idk why but i could do that everyday for the rest of my life… mycelium are soooo fucking fascinating. I had some pan cyan spores i was isolating and had a isolate that would literally eat trichoderma for breakfast but the fucker wouldnt fruit for shit. Worked on it for months. Cloning is fun too. I kinda miss the tinkering with it but will be to busy to take that up again
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