Mr.jiujitsu
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Silver Thiosulfate – Plant Tissue Culture Protocol
Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate into 100 ml of water. Store the stock solutions in the dark until needed to prepare the STS.www.sigmaaldrich.com
"Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate (Product No. S7026) into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate (Product No. S7276) into 100 ml of water. Store the stock solutions in the dark until needed to prepare the STS.
In general, the STS is prepared with a molar ratio between silver and thiosulfate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of
[Ag(S2O3)2]3-, the active complex for ethylene effect inhibition.
Prepare a 0.02 M STS by slowly pouring 20 ml of 0.1 M silver nitrate stock solution into 80 ml of 0.1 M sodium thiosulfate stock solution. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended."
Just spend a few bucks and do it properly. It's faster, easier, and 100%
Silver Thiosulfate – Plant Tissue Culture Protocol
Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate into 100 ml of water. Store the stock solutions in the dark until needed to prepare the STS.www.sigmaaldrich.com
"Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate (Product No. S7026) into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate (Product No. S7276) into 100 ml of water. Store the stock solutions in the dark until needed to prepare the STS.
In general, the STS is prepared with a molar ratio between silver and thiosulfate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of
[Ag(S2O3)2]3-, the active complex for ethylene effect inhibition.
Prepare a 0.02 M STS by slowly pouring 20 ml of 0.1 M silver nitrate stock solution into 80 ml of 0.1 M sodium thiosulfate stock solution. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended."
Just spend a few bucks and do it properly. It's faster, easier, and 100%
It's a standard practice in the horticulture industry, tested and true for...I don't even know how long. The thiosulphate is cheap as can be and the nitrate mid range. But you don't need much, so don't order a bunch thinking you'll have a stockpile or anything. They do have shelf lives.Pretty cheap to run. I’ll give it a try, but looks legit- thanks
Awesome I’m excited to give it a run. Ordered. I don’t know how I missed this. Thanks again.It's a standard practice in the horticulture industry, tested and true for...I don't even know how long. The thiosulphate is cheap as can be and the nitrate mid range. But you don't need much, so don't order a bunch thinking you'll have a stockpile or anything. They do have shelf lives.
But yea, remember, just the tip!
A whole branch will give you hundreds of male flowers, so unless you want a dust storm, less is more
So, by just the tip, you mean to spray just the tip of the plant, and that is the only part that will "anomolize" ? For lack of a more appropriate word in my head at the moment? I am toying around with the idea of doing this myself in the coming month rather than pay the outrageous prices for 3 fem seed packs and risk them being sent and not damaged or worse. how easy would it be to accomplish something in a tent on the other side of the house and not affect my flower room? How easy is it to get started? I have a decent knowledge of plants and basic horticulture skills, so I wont be learning another language, just how to conjugate a few verbs, right?It's a standard practice in the horticulture industry, tested and true for...I don't even know how long. The thiosulphate is cheap as can be and the nitrate mid range. But you don't need much, so don't order a bunch thinking you'll have a stockpile or anything. They do have shelf lives.
But yea, remember, just the tip!
A whole branch will give you hundreds of male flowers, so unless you want a dust storm, less is more
Well, if you just spray the tip of a branch you'll end up with dozens of males flowers from that spot alone.So, by just the tip, you mean to spray just the tip of the plant, and that is the only part that will "anomolize" ? For lack of a more appropriate word in my head at the moment? I am toying around with the idea of doing this myself in the coming month rather than pay the outrageous prices for 3 fem seed packs and risk them being sent and not damaged or worse. how easy would it be to accomplish something in a tent on the other side of the house and not affect my flower room? How easy is it to get started? I have a decent knowledge of plants and basic horticulture skills, so I wont be learning another language, just how to conjugate a few verbs, right?
You might end up with a few self polinnating if they are like directly above a female flower, without pollinating insects you either need a lot of male flowers to open or, you guessed it, you get to do the dirty work. Well, I guess it's not dirty if you like to watch I suppose, but don't be a pervert.Will these self pollinate or should I still run another and paint brush pollenate ?
You might end up with a few self polinnating if they are like directly above a female flower, without pollinating insects you either need a lot of male flowers to open or, you guessed it, you get to do the dirty work. Well, I guess it's not dirty if you like to watch I suppose, but don't be a pervert.
I think it can also e made by electrolysis… A car battery and some silver, but I don't know the correct way to determine the concentrations of the silver solution. I used to enjoy messing with electrolysis when Nickle coating pennies and stuff like that.
His method is much more accurate and easier... just mixing some chems together instead of a Frankenstein setup. It is also easily available online and is pretty cheap. Just be careful as it is light sensitive and can break down if exposed to too much light.So that’s what I have been making, I have been producing 40ppm. But after doing more research and finding that @ahemait has some legit science backing up his claims I’m going to use both. Honestly though it appears pointless as the solution ratio he gives makes it cheap, easy to make, and from what multiple sources say 100% effective. Still I’ll see how the CS works, and most likely gift or sell the remaining to some friends who like it for health and sterilization
His method is much more accurate and easier... just mixing some chems together instead of a Frankenstein setup. It is also easily available online and is pretty cheap. Just be careful as it is light sensitive and can break down if exposed to too much light.
I like it for the magical electrolysis process, which I've messed with as a kid, not long after Franklin discovered electricity! It's the fun of it and the ability to do it... not necessarily the end result, but the attempting is the fun part.I like to make it build as much as I can. I enjoy experimenting and developing for my own self centered reasons. That being said I like pulling out the beakers and mixing up a batch - it’s fresh and I can feel confident that I know what’s in it.
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