Graywolf said:

I've certainly looked hard at the Rotovape and it would be easy to engineer one, using a simple rotary union, but most of our oil goes into sublingual medication, so we just cook off the solvent and decarboxylate in the same step, making vacuum un-necessary.

Our simple vacuum chamber does an stellar job in the small quantities of carboxylic acid cannabis products that we produce by thin film vacuum purging and it is easy to remove afterwards.

A Rotovape is on the wish list, but the used Perkins Elmer Gas Chromatograph that we just picked up was way higher on that list, because we are tired of shooting blind and relying on only anecdotal information. I under stand how a GC works, but there is zero chance that I could ever engineer one myself.

Next highest on our wish list, is the rest of the equipment that our biochemistry student requires for his tissue culture, synthetic seeds, DNA sequencing experiments, and fraction distillation separating the stream constitutes by refractive index.

I would grab a Rotovape at a price I couldn't refuse, but in retirement am on a fixed income and have to pick and choose toys. We hope that eventually Skunk Pharm Research lease program income will pick up that slack and start to support more and better toys, so I can start to spend my own monthly mad money allowance on other thangs.

Maybe take my tolerant and supportive wife mate on a wild weekend wearing golf balls as big as diamonds for instance.

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squiggly said:

I would give an arm and a leg to be "your student". Can't describe how badly I'd love to be able to legally bring cannabinoids and terpenes into the lab and run testing.

I've lately been reading up in these experiments where tobacco has been engineered with some avian DNA and over expressed (placed adjacent to a gene which is copied often), which turned terpene production up 1000%.

I want so badly to have the technology in my hands to do this (and the team of people to run a genome, etc., that it'd take). I essentially already have the know how--the instructions are right there. All I need is the genome, a plasmid, and the right tools.

I've got so many little experiments on my wishlist--and no way of doing any of them.

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Mississip Hip said:

are you saying they mixed bird DNA and tobacco DNA?.....:confused:

I dont know what avian means.

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Graywolf said:

Before registering Skunk Pharm Research, LLC, we used that equipment set up in my garage for our initial research. Squiggly didn't mention it, but a soxhlet makes killer coffee!

We've made a number of cannabis esters using this equipment, concocted potions, and last Christmas made some popular cannabis soap and massage bars for stocking stuffers.

With the inclusion of a separatory funnel, I also use this equipment to clean up other extractions, and have extracted essential oils from hops, for a hops farmer looking for a way to preserve and market his surplus harvest.

The glassware is all basic and the foundation to any number of chemical procedures and experiments.

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squiggly said:

This is exactly what it means.

essentially the bird's body needs waaaay more of the chemical which is a precursor to the terpene synthesis pathway than a plant does and has proteins which can produce a shit ton of it. They gave the genes coding for these proteins to the tobacco and the result was a LOT more terpenes.

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squiggly said:

I've lately been reading up in these experiments where tobacco has been engineered with some avian DNA and over expressed (placed adjacent to a gene which is copied often), which turned terpene production up 1000%.

I want so badly to have the technology in my hands to do this (and the team of people to run a genome, etc., that it'd take). I essentially already have the know how--the instructions are right there. All I need is the genome, a plasmid, and the right tools.

I've got so many little experiments on my wishlist--and no way of doing any of them.

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Mississip Hip said:

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caregiverken said:

you wanna GMO weed? o_O
with a lizard maybe?

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caregiverken said:

the Marlboro's I smoke are GMOed? :confused:

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Graywolf said:

Here are some pictures of some of the glassware discussed. I also threw in a soxhlet.

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