http://nfe.localgarden.us/index.php?title=Indigenous_Micro_Organisms#How_to_collect_IMOs
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The IMOs can be collected by various methods. It can be collected from hills and mountains using steamed rice with low moisture ( hard- boiled ), decomposed leaves and bamboo stumps. It is possible to collect, to a certain extent, particular types of microorganisms.
Collecting from forest
1. Fill a wooden lunch box (or any natural material like hala basket etc ) with hard- steamed rice. The rice should not be packed deeper than 7 cm (about little less than 3 inches ). This is for air supply. Without sufficient air supply, anaerobic microorganism will be collected. Anaerobic microorganisms are more commonly recommended.
2. cover the lunchbox with rough paper ( so air can get through )and tie it ti the box rubber band.
3. Bury the lunchbox in the local bamboo field or decomposed leaf molds in the hills. Cover with leaves. Be careful that the leaves press the paper to touch the rice surface.
4. Cover plastic sheet on the leaves above the lunchbox to prevent rain from getting through.
5. At 20 C or 70 F, it will take about 4 to 5 days ( faster when hotter ) for the IMOs to fill up the box. Move this rice ( this is called IMO 1 ) to the clay pot.
6. Mix crude ( or brown ) sugar with the IMO 1 in 1:1 ratio ( this is called IMO 2 ).
7. Cover the clay pot with paper and tie with rubber band.
Collecting from leaf mold
1. Go to the hills forests, valleys, you will find leaf molds full of white hypha. Collect this IMO mold. Deciduous tree forests are better because evergreen forest have less microorganisms.
2. Dip hard-steamed rice in solution of FPJ ( fermented plant juice) diluted 1,000 times. Warm it then leave it to cool.
3. mix this rice with the leaf mold. Leave for one night.
4. mix this compound into rice bran (or wheat mill ) for propagation. Cover the rice bran with straw to promote IMO growth.
5. You can add FPJ ( fermented plant juice ), FAA ( fish amino acid ), mineral A, etc. to boost process.
Collecting from bamboo stumps
1. Choose a bamboo tree in the center of the bamboo forest. Slice the trunk at 10 cm from the ground obliquely. Cut it so that inner part is lower than the outer part so that the bamboo juice will not leak out.
2. Fill in hard-boiled rice in the bamboo cavity: rice should be higher than the brim.
3. Put a wooden lunch box C cedar ) over the stump.
4. Cover the box with leaves.
5. Cover with plastic sheet and then put a weight on top so it won't fly away.
6. In 3-5 days, red, white, yellow, black and all sorts of bacterias will have gathered. Juice from bamboo will also be collected.
7. Cut the stump. Pour the rice in clay pot ( this is IMO 1 ).
8. Mix crude ( or brown ) Sugar with IMO1 in 1:1 ratio ( this is IMO@ ).
Collecting from rice-paddy
1. After harvest, cover the rice stump with rice lunchbox immediately after cutting. The lunchbox faces downward.
2. Cover with steel wire net to prevent mouse.
3. Cover with plastic sheet to prevent rain.
4. After approximately one week, IMO will have gathered.
5. Pour the rice clay pot ( this is IMO1).
6. Mix crude ( or brown) sugar with the IMO1 in 1:1 ratio ( this is IMO2).
7. Cover the clay pot with paper, and tie with rubber band.
When you collect microorganism from rice paddies, in contrast to the microorganisms collected from bamboo forest or leaf molds, you can get a lot of anaerobic microorganisms. In particular, you can collect a large quantity of ; carbohydrates; and bacillus subtitles that disintegrates strong fibers such as straws and straws and reeds. Theses two mocroorganisms have outstanding decomposing power.
But when the fermentation temperature goes up above 70 C, they not only convert protein into amino-acid into ammonia. In such case, the nutrients are lost to the air. Therefore, the fermentation temperature should be maintained at below 50 C. Lactic acid bacteria feed on sugar and amino acid made by bacillus licheniformis and bacillus subtilis.
Adding lactic acid bacteria can lower the temperature.