rootsnshoots
- 141
- 28
I think I came across that a bit ago.. If i remember right they were getting hermies after forming callus? If you could refresh my memory with the link?
I think I came across that a bit ago.. If i remember right they were getting hermies after forming callus? If you could refresh my memory with the link?
rootsnshoots
- 141
- 28
Awesome!! Where did you get it? Whats the deets?
T
thierry
- 2
- 1
cool interresting discution
T
thierry
- 2
- 1
I'm not sure anderstand everything so sorry for stupide question :
Are you sure you are doing callus multiplication and after shoot regeneration ?
Are you sure you are doing callus multiplication and after shoot regeneration ?
pork
- 197
- 28
digdugdank
Select Genetics
- 327
- 93
Bioguy, I got a question for you! The study you read, were they using callus or shoots? Or does it not matter? I'm so new to this and want to ask the right questions! Thanks digdug! :)
digdugdank
Select Genetics
- 327
- 93
Same kit I got :)
B
bioguy
- 9
- 3
Here is a link to the post. Not mine just a link back to what I think is the most comprehensive outline of TC/MP
https://www.thcfarmer.com/community/threads/micropropagation-of-cannabis.19773/
Looks like the used a variety of explant material. Which one hermied and which ones did not I'm not sure with a re-read that I just don't have time for. Hope this helps someone.
https://www.thcfarmer.com/community/threads/micropropagation-of-cannabis.19773/
Looks like the used a variety of explant material. Which one hermied and which ones did not I'm not sure with a re-read that I just don't have time for. Hope this helps someone.
B
bioguy
- 9
- 3
Another thought. Soil nutrients and environment can be manipulated to increase the percentage of males/females when sprouting seeds. This is evidence that female plants are to some extent an environmentally triggered phenotype within a broader genotype. Tissue culture preserves genotype not phenotype even thou we would expect (and have seen) preservation of both.WHAT OTHER TRAITS MIGHT ALSO BE ENVIRONMENTALLY TRIGGERED
I would be very curious if the m/f ratio in seeds is the same from the cultures (or if it can be manipulated). In the end this could lead to profound understanding about phenotype expression.
If a plant is born female but under different conditions would have otherwise developed male is it then also more likely to hermie. In the opposite case would the female be a better candidate for sex reversal...I mean a male plant that "could have been female" seems more likely to pass on the best flowering genes. Theoretically, we could use these "either sex plants" to pollinate our stash knowing all we had to do was increase nitrogen to get all females in the seed stock. But without TS we would have no way of knowing which plant might have been environmentally manipulated into its current sex.
I would be very curious if the m/f ratio in seeds is the same from the cultures (or if it can be manipulated). In the end this could lead to profound understanding about phenotype expression.
If a plant is born female but under different conditions would have otherwise developed male is it then also more likely to hermie. In the opposite case would the female be a better candidate for sex reversal...I mean a male plant that "could have been female" seems more likely to pass on the best flowering genes. Theoretically, we could use these "either sex plants" to pollinate our stash knowing all we had to do was increase nitrogen to get all females in the seed stock. But without TS we would have no way of knowing which plant might have been environmentally manipulated into its current sex.
B
bioguy
- 9
- 3
Dont forget H202 kills fungal spores but not fungus. A second sterilizer is needed when using H202.
oxanaca
- 322
- 63
anyways if shoots and roots decides to come back well have a little grow off. otherwise im gonna jack this thread some more. lol
oxanaca
- 322
- 63
damn steep price $246
for $20 more you could build my flow 24x18" flow hood.
seems like a rip off
fuck magenta tc enclosures, id rather use quart jars, with 70mm clear polypropylene disks.
sorry for the rant
oxanaca
- 322
- 63
im thinking of trying the no agar, liquid medium route.
i will need to build a rotator, if i intend to do this though. but it seems like it could solve alot of the problems agar causes. such as too hard/soft, insufficient dissolved oxygen, build up of waste chemicals next to a plant, no need to wash off agar prior to transferring to soil. although you lose your ability to easily spot bacteria fungus.
kind of reminds me of liquid culture in another hobby, know what i mean
i will need to build a rotator, if i intend to do this though. but it seems like it could solve alot of the problems agar causes. such as too hard/soft, insufficient dissolved oxygen, build up of waste chemicals next to a plant, no need to wash off agar prior to transferring to soil. although you lose your ability to easily spot bacteria fungus.
kind of reminds me of liquid culture in another hobby, know what i mean
oxanaca
- 322
- 63
damn is this thread dead? i was hoping i would be able to talk media formulations and share info
ill keep checking in, come back when you can shoots and roots
ill keep checking in, come back when you can shoots and roots
rootsnshoots
- 141
- 28
Sorry for my absence fellas!
Quick recap, I quit the dispensary I was working for and am back to doing my own thing. (Finish carpentry, custom cabinets, high end finishing....and growing goods.)
So I built this bad boy!!
and here's how you do it;
Get a sheet of white melamine, and cut to size on table saw
rig up the dado
Glue it up
Set the filter. I used aluminum angle to squeeze the filter in. Super clean and durable.
Set the fan, and let silicon cure for a couple days
Now lets use this baller box;
clean explants
cut em to size
walla!
Quick recap, I quit the dispensary I was working for and am back to doing my own thing. (Finish carpentry, custom cabinets, high end finishing....and growing goods.)
So I built this bad boy!!
and here's how you do it;
Get a sheet of white melamine, and cut to size on table saw
rig up the dado
Glue it up
Set the filter. I used aluminum angle to squeeze the filter in. Super clean and durable.
Set the fan, and let silicon cure for a couple days
Now lets use this baller box;
clean explants
cut em to size
walla!
oxanaca
- 322
- 63
good job dude
looks like you have the same size filter it is a 18x24" right?
i want to join in on the fun ill probably not post any cannabis publicly though,
my balls aren't as big as yours:cool:
looks like you have the same size filter it is a 18x24" right?
i want to join in on the fun ill probably not post any cannabis publicly though,
my balls aren't as big as yours:cool:
rootsnshoots
- 141
- 28
oxanaca
- 322
- 63
not at the state level anyways
oxanaca
- 322
- 63
what ever became of this dude?
W
western5
- 5
- 1
Marijuana from test tubes is now available on Amazon. It is a book that has an illustrated step by step guide for TC of cannabis
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