rootsnshoots
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Great thread! Are you forming a callous first and then going the way of roots and shoots (no pun intended!)?
Nope going for shoots here first. Actually I don't really know what I'm doing at all. But hey sure is fun.
Great thread! Are you forming a callous first and then going the way of roots and shoots (no pun intended!)?
Nope going for shoots here first. Actually I don't really know what I'm doing at all. But hey sure is fun.
Good stuff bro! great to see how your experiments are coming along. Really makes me want to give it a crack, though would need some serious investment into equipment before I could do that.
Keep us updated!
Can you get growth from just cellulose?
Whats the best to use for explants?
I'm almost positive the contamination came from lack of cleaning the explants. But I did have one that contaminated the agar away from the explant. Looks like Trichoderma. That one was probably a floaty..... Shmuv...
Dead, dry cellulose will not be propagatable, no. For most plants, you want meristematic tissue- stuff that's growing the fastest, usually a growing tip of some sort- and that includes floral material. For most plants, this includes nodes; not cannabis, best as I know. The literature seems pretty clear in that most people use leaf material.
Contamination away from the explants is usually airborne (or coming from the hands, that sort of thing). Disinfestation of tissue just shy of killing it may be required; there are all sorts of tricks and techniques, most of which involve fairly complex liquid techniques. However, one fairly simple trick is to use an ultrasonic generator- the cheaper, the better. More powerful units will also destroy plant tissue. So, while the plant matter is in a disinfection solution, that container is placed in the ultrasonic cleaner (usually a cheap jewelry cleaner) for a few seconds- enough to help collapse air bubbles and enhance penetration.
Great thread Rootnshoots. Micropropagation is absolutely fascinating.
Have you found your experiments to be costly so far, including the cost of equipment, hormones, etc.?
Have you tried using growing tips as material?
I figger no dead stuff. Was just wondering if taking the inside of the stalk will be more contam free, as its not exposed to air. I know when cloning from mushrooms you take your culture from the inside of the stem. As it is contaminant free.
The tdz article used nodal segments with great results