Mushroom thread

Dirtbag · Dec 26, 2020

1diesel1 said:
All rite,

Cubensis | Doc's Dank Spores

www.docsdankspores.com

Is taking the winter off.
I need to source my spore syringes elsewhere for now.
Ideas??

Sporeworks looks awesome. Thats where Id be buying from if i lived in the US.

Sporeworks.com

Spore Works has been supplying quality rare and exotic mushroom spores since October of 1998. Our primary focus is providing our customers with the highest quality spore and culture material, and the widest available selection of varieties and species.

sporeworks.com

amekins · Dec 26, 2020

I believe I saw @beluga post positively about these guys

Sporeworks.com

Spore Works has been supplying quality rare and exotic mushroom spores since October of 1998. Our primary focus is providing our customers with the highest quality spore and culture material, and the widest available selection of varieties and species.

sporeworks.com

Dirtbag · Dec 26, 2020

Amazing how many more pins showed up today.
Anyone have any advice on how to best handle the next few days? I havent misted or fanned these at all. I dont even open the lid except to snap the odd pic lol. I do have a cieling fan going set to low for some gentle air circulation in the room theyre in. Thats it though..
Each tote has 8 x 1/4" holes, 4 on each side covered in micropore tape. And the boxes seem to have a bit of condensation, but not too much. Plenty of really tiny water droplets on the surface of the cakes. And i see shitloads of more little brown spots about to pin. This cake is gonna be just covered.

I also think i might have spazzed out a bit prematurely regarding my g2g jars...
Theyre coming along now. I think the condensation was from the mycelium respiring. The grain looks dry and its colonizing rapidly. The Agar is what i seem to be having the most trouble with, but ill keep at it. Im hooked now lol.

Actually not bad considering I only innoculated these jars 6 days ago..

1diesel1 · Dec 26, 2020

Dirtbag said:
Anyone have any advice on how to best handle the next few days?

Walk away for 24 hours and relax:)

Dirtbag · Dec 26, 2020

1diesel1 said:
Walk away for 24 hours and relax:)

Sounds good to me.
They made it this far. May as well keep not doing what i havent been doing.

1diesel1 · Dec 27, 2020

First soak on this tub,
I’m seeing a bit of contamination where the stumps are located and also premature mushrooms, but overall everything is looking good............I think:)?

1diesel1 · Dec 27, 2020

1diesel1 · Dec 27, 2020

1diesel1 · Dec 27, 2020

1diesel1 · Dec 27, 2020

I will devise a way to remove the entire mushroom without pulling chunks of tub material when harvesting fruits.

amekins · Dec 27, 2020

1diesel1 said:
I will devise a way to remove the entire mushroom without pulling chunks of tub material when harvesting fruits.

How about a guitar wire loop?

chemistry · Dec 27, 2020

Ice cream scoop?

1diesel1 · Dec 27, 2020

I actually used a regular spoon, just to see how it would work, edge was to thick though.
It would work perfect if it was sharp like a knife

chemistry · Dec 27, 2020

Grapefruit Knife. lol

Fudge · Dec 27, 2020

Apple corer with the end cut off so it's a semi-circle like the rest of it

Dirtbag · Dec 27, 2020

I might just try the wiggle and twist method of removing them when the time comes.

beluga · Dec 28, 2020

I've missed some stuff here.
Holidays just aren't holidays for me without an extra healthy dose of fuckin' off to remedy my raging case of the fuckits.

Jimster said:
Got a question on making the jars up. I saw the process making the medium and topping it with vermiculite before steaming for a while. Would it be feasible to sterilize them in an oven instead of a pressure cooker type of thing? I know that you would need to be careful with the moisture level, more so than steaming, but it sounds like it should work out OK. Any thought from those who have ventured into this process?

Dry heat sterilization is really ineffective in this type of scenario where you need to penetrate into a three-dimensional core and keep the temperature there high and stable.
Steam/water carries heat far more effectively and can maintain those stable high temperatures.

The only thing the oven is useful for is sterilizing two-dimensional surfaces like those of foil for taking prints or glass petri dishes.

Dirtbag said:
Anyone have any advice on how to best handle the next few days?

1diesel1 said:
Walk away for 24 hours and relax:)

X1000.
Although they move at a really observable rate, it's always best to leave 'em be.
Clear lids make the gawking less intrusive.

1diesel1 said:
First soak on this tub,
I’m seeing a bit of contamination where the stumps are located and also premature mushrooms, but overall everything is looking good............I think:)?View attachment 1072277

Looks great!
As far as the 'aborts' and contamination over the stumps, it can be expected until you've got your culture cleaned and relatively isolated and your substrate preparation (field capacity) dialed in. Were you to gouge them out by plucking or grapefruit spoon, you would just leave even more opportunity for contamination to take hold.

Keep in mind that mycelium and mushroom tissue serve as a shield that encapsulate their food source/rhizosphere.
So long as there's full coverage of just one colony, then all the contaminating cultures are simply just stuck on the mycelium (or mushroom tissue), unable to germinate or replicate because there's nothing to actually latch onto or feed off of.
So, whenever we compromise that shield, we compromise the integrity of the the colony and thus leave gaps for contamination to find nutrient/habitat sources with which to replicate or germinate on.
There are, of course, predatory species like trichoderma that can do their own compromising of that shield, so we do our best to eliminate those competitors from the get-go with various preparations... one of the simplest being the use of mostly-inert substrates like coir.

Dirtbag · Dec 28, 2020

Picking up speed now, damn this is fun to watch!

1diesel1 · Dec 29, 2020

Tubs 2-3 beginning:)

Dirtbag · Dec 29, 2020

1diesel1 said:
Tubs 2-3 beginning:)View attachment 1073073 View attachment 1073074

Noice man! What size totes are those?

Mushroom thread

Mushroom thread

Dirtbag

Cubensis | Doc's Dank Spores

Sporeworks.com

amekins

Sporeworks.com

Dirtbag

1diesel1

Dirtbag

1diesel1

1diesel1

1diesel1

1diesel1

1diesel1

amekins

chemistry

1diesel1

chemistry

Fudge

Dirtbag

beluga

Dirtbag

1diesel1

Dirtbag

Privacy & Transparency

Privacy & Transparency