1diesel1
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So, spore syringes are inherently dirty. But that doesn't mean that direct inoculation can't work, but it's a much bigger uncertainty. I did direct inoculation for a while with some success but also a lot of green.
Couple questions, (I think you may have posted, but I suck at going back and forth on my phone):
- Did you vent your PC for 10 minutes once it had a full stream of steam?
- How long did you PC?
Your grain looks prepped quite well, but that doesn't make or prevent contamination... it had to be introduced somewhere.
Couple notes:
- Glove boxes can create a positive air pressure within them that will force stuff into your media. I would pop those gloves out and try it in the Still Air Box form. Deliberate, smooth movements so to not disturb the air much.
- Did I see that you had tape over your grain jar lids? They have to be able to release steam in order to properly sterilize, so there should be some form of air exchange.
Any extra details you can think of, I'm happy to assist in troubleshooting.
what is the proper process of grain jar sterilization?