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ilaughlast
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i am not attempting this as part of a strain hoarding endeavor. i am trying this to figure out if mother stock of rare plants can be saved rather than ditched
Is this just a thought or do you have any test results to back this up?it isnt a breeding thing or even dna/rna altering
the fact is fusarium attacks the plant by overloading if with specific homones and acids. It attacks the cell walls, essentially taking all our prized genetics and ripping them down to their main frame as a 5% thc yielding hemp plant.
It is not gentic drift
It is not TMV
And it is not permanent
the key will be a battle at the microbial level .. beneficial microbes v the bad guys .. perhaps some new engineered species of microbe... but i hope they hurry up loltrue but it would lend us a clue as to how to solve this probl;em in cultivars that would otherwise be lost.
me and @We Solidarity are on the same water @true grit
120 ppm or .2 ec out the tap.
it isnt a breeding thing or even dna/rna altering
the fact is fusarium attacks the plant by overloading if with specific homones and acids. It attacks the cell walls, essentially taking all our prized genetics and ripping them down to their main frame as a 5% thc yielding hemp plant.
It is not gentic drift
It is not TMV
And it is not permanent
this........the key will be a battle at the microbial level .. beneficial microbes v the bad guys .. perhaps some new engineered species of microbe... but i hope they hurry up lol
i have a theory i would like to test.
freshly divided primordial undifferentiated apical meristem cells as they emerge are free from the host plants viruses in most cases.
these cells as they emerge are said to be free of the host plants pathogens for dozens of hours.
in strawberry nursery stock, it is the standard for clearing viruses and other pathogens to perform a special type of in vitro tissue culture called MICROtip culture.
you may be familiar with MACROtip tissue culture and have likely seen the home tissue culture kits for sale online.
this is not quite the same however very similar. this requires a very small sample (<1mm) of just the emerging cells of the meristem. it is usually done by someone with practice in this precision cut done under at least 80x magnification. the explant is then sterilized and placed in culture in a medium containing shoot induction hormones in a petri dish much like a macrotip culture. the incubation time is much longer and is therefore much more susceptible to infection/contamination of petris. while this is fairly impractical at home it can be done in a lab setting by a trained tech with the right equipment and knowledge of protocols.
i think if this is a pathogen from bms be it viral fungal bacterial etc it could be cleared and resume regular growth given it is reintroduced to a clean environment. i am currently trying to locate a confirmed dud by a reliable source with an intimate knowledge of the syndrome on which i can conduct this experiment and feel confident im working with the correct problem and not a new growers poor growing style instead of the actual duds.
thoughts?
woah........ sketchfussarium oxysporum was specifically developed to eradicate drug crops .. i don't have the links on this computer but it was developed in the 60's at a biotech lab in montana and then in the 70's uc berkeley reported success in the mass production of fussarium oxysporum for an engineered bio-weapon against drug crops.. fast forward to modern times uc santa cruz professor examines chemtrail content and reports weaponized fussarium oxysporum as a part of the spray content.. effect to californias food production expected to top a 30% reduction says uc santa cruz professor.
my prediction is that we will see this problem get far worse and then gmo fussarium resistant cannabis wont be far behind .... wait until a few huge investors lose a few 1000 light grows they will beg for gmo'ed weed ...
i have seen this problem for nearly 20 years it is not new but it is spreading faster because of the practice of trading cuts and the shear number of green rushers ...
very nice i think its the only way to at the very least preserve infected strains. id love to hear what results you get. thanks for sharing your set up. very cool. im glad that others are working on this as a solution too.Yes, I plan on using Meristem Tissue cultures. Here's a picture to get an idea on how it works. Even if I can't figure out what is causing it, I can at least fix the problem. All my strains are going into tissue culture. Everything will be killed off and sterilized.
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Some symptoms. Random leaf twisting.
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Pictures of my past work with my flow hood and agar cultures.
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Pictures of my old flow hood when it was unfinished.
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New flow hood that is getting built, it's unfinished. View attachment 407933
very nice i think its the only way to at the very least preserve infected strains. id love to hear what results you get. thanks for sharing your set up. very cool. im glad that others are working on this as a solution too.
I wonder if this is more common in soil beds or in reused soil vs single plant containers and fresh soil.im not sure of everyones grow methods here but it doesn't seem to be a lot of hydro guys talking about this?
well because you said to,i did go back and read lol.do you see a lot of hydro growers in this thread with this problem?the ones I see pics of are in soil, coco or beds of some variation.other than cap who else has had this dud problem in hydro?
someone posted tissue culture of what looks like mushrooms and theres a page of tc talk and a page of speculation of tmv.but a couple mottled leaves,twisted growth,or larfy buds could be many things.dalae632 has actual duds where some plants just had one or two branches that def look full on dud.i just think some guys are calling poorly grown plants duds where other growers who have great looking rooms with just a few plants that have an obvious new problem.im just curious at this point,i don't do cuts and I grow outdoors.but I do start my plants indoors and I def want to avoid this dud issue if possible.but I have been around forums and read a lot of big room threads.maybe some guys are keeping marginal cuts or runt seedlings that would be culled by other guys and trying to grow those runts full term and a dud is the result?seen a few big grows here,jackmayoffer or waayne have these duds? or maybe those guys spot them early on and cull them as runts and never seen a dud on chop day? maybe with all the complaints on the net about fungus gnats in soil and coco these companies started using something chemically to combat the bugs that are affecting certain strains idk,