Ricksauce
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I also do not see dudding
I've had moderate success with TC. As long as sterile procedure is followed and you have a proper hormone ratio it's not too bad.. So I can culture out the dudding with the plants Im about to get?That's our next step. I've got a culture lab for in-house fungal diagnostics but that's easy compared to this. Meristem tissue culture hasn't been necessary for me until now. It's tough. It'll take a few months to get operational but we've pulled the trigger. I'll update. I still want an ID, but the answer is the same regardless of what virus this is. We're go for live tissue culture.
There are so many questions to be answered here, I don't know. I'll be operational in weeks, results will take months. If this is viral, TC is the most viable solution. Heat therapy looks promising, sort of. If you're set up to do that, run it and let us know how you do. It's tough to do it right. Lot of moving parts and it's unforgiving. Antiviral cleaners are only the beginning.I've had moderate success with TC. As long as sterile procedure is followed and you have a proper hormone ratio it's not too bad.. So I can culture out the dudding with the plants Im about to get?
I'm not growing right now but when I start back up with new stock from homies what precautions should I take.. All equipment should be cleaned with an anti viral cleaner?
Autoclave or pressure cookeri already have a flow hood and a lot of organic chemistry equipment.. like i said ive produced roots and shoots with meristem and callous culture (carrot culture.. roots)
edit:
just read the link, yeah pretty much got all that covered.. i still have a lot of my old hormones i used but if i recall right i think they had a 30-60 day shelf life so ill need more of that but i have plenty of sterile agar and nutrient mix.
the only thing i could see makign things easier is probably a easier fast track way to clean the vessels when done.. (UV sterilization or some equiv)
lol theres the word i was thinking of! (autoclave)Autoclave or pressure cooker
So your saying the title white bumps are CaC204?That's what led me to look at root zone fungal infections to start. I pulled my hair out looking for a fungus - for maybe two years - something that causes duds. This started because of the absence of anchor roots in clones and plants. So the generic dwarfing and runting of the plants appears to mimic some sort of nutrient lockout. That's why I looked at the xylem and was surprised to find it all clogged with spiky balls - later identified (By AJ, thanks AJ!) as CaC2O4 druse. Now I'm getting into unproven ground. It's possible I'm completely wrong about this. I believe the xylem is impaired. That works both ways - less water/nutrients up, less water/waste down. Makes sense. That would explain the poor root development and overall malaise of the plant. So my model might look like this:
Viral load high -> Plant Immune Response -> Accumulation of CaC2O4 druse -> Xylem impairment -> Duds
There's an equation I'm working with that looks like this, simplified:
If the rate of mitosis in the plant ≥ x(rate of viral reproduction) then the plant is asymptomatic
If the rate of mitosis in the plant ≤ x(rate of viral reproduction) then the plant shows duds
This would explain why excellent growing conditions produce fewer duds. Anything you can do to either impede the reproduction rate of the virus, and/or, increase the rate of cellular reproduction in the plant, increases the plants chance at surviving without symptoms.
I think a possible source of uninfected stock might be farmers who have old mother stock. By my logic that can't exist duds free. Old moms necessarily eventually succumb to the disease. Also old seed stock - say pre 2005 might be a solid source of clean genetics.
Again, this is all conjecture and I'm happy to be corrected if anyone has evidence that disproves anything I say. Bring it up, lets fix this. If it plays, it plays. This is interesting by the way.
@bulldog aren't those pics from last spring before the mite infestation?
This is pretty much a must-watch for anybody following this thread closely. The Q&A at the end is worth the wait. All of it lands pretty much on the money. 45 minutes well spent.If price is not a concern for testing, I think I might be able to help with some ideas. Anyone serious shoot me a pm and we can discuss. This could be a big project and take way more time than anyone has to spare but the nitty gritty of the data may be worth it.
Great video, that's a class I would not be bored at all in.This is pretty much a must-watch for anybody following this thread closely. The Q&A at the end is worth the wait. All of it lands pretty much on the money. 45 minutes well spent.