I will go into some new points and gene sequences that I havent mentioned before. I sent the complex stuff off to some peeps that wanted exact gene location. I dont get that stuff. That was just too much for this thread, but I'll break down some proof as requested.
This point is that when we eliminate a (Y) chrome we lost DNA. In a cattle yard we'll have 1 bad azz bull who carries these traits. The bull carries genes of vigor, endurance, size and on and on... But I need to prove that the DNA helix'S , one in the (X) chrome and one in the (Y) chrome contain SPECIFIC DNA that define our traits. Once we prove that, we understand that feminized organisms are weak and frail and gay/hermy sissy boys and to survive some of the girls can get rough and tough.
Again in a natural punnet breeding chart, (bred in the wild) , will normalize the crop back to more vigorous crop and strain.
The work presented here describes a single DNA fragment generated by one decamer primer that appears to be closely associated to the male phenotype in different cannabis cultivars and accessions and its transformation into a sequence-characterized amplified region (SCAR) marker allowing a rapid and specific identification of the male sex.
So Ive explain what I know about the MeGI and DOI gene sequence in cannabis and this topic leads us into Male and Female specific genes and their traits. But first we need to remeber that the MeGI and DOI genes are found in both DNA Helix in each chrome X and Y. So with that said we'll always have the hermy/gay genes and traits.
Some of may already know this but when you Feminize a Female organism, Plant or animal you will lose the Y chrome. So here is fun fact to ponder. Why is the cannabis hermy's and hermy in our kids similarly abundant? Gibbs, and other hormones are sprayed on food and cannabis crops. hmmmm...
so we have found cannabis DNA genes that we have found on ONLY the Y chrome, Male plants.
OPA8400 a con sed that has other Male specific DNA genes and are almost never found in female plants. more specific below. We'r finding that the MADC2 and even the MADC1 DNA gene sequence are relevant in many traits Male traits. I think and dont quote me on this but I suspect that MADC1 is not a accurate male associate and might have Female influences also.So even tho MADC1 is thought to me a Male DNA only, it has been found that is not and I Think it will be found in Females more often than currently documented.
The PROOF is in the RAPD band of the DNA slabs. This proves that eliminating the Y chrome genetically modifies the organism to Not have these traits anymore.
it was found that the decamer primer OPA8 (5@-GTGACGTAGG-3@), when tested on the dioecious cultivars , produced a band of approximately 400 bp that appeared to be male-specific. To check the possibility that the OPA8400 band was linked to the male phenotype, we screened a group of 167 plants belonging to seven commercial cultivars (5 dioecious and 2 monoecious) and seven accessions from germplasm banks or collections for the presence or absence of this 400-bp marker. The results are summarized shows the amplification patterns produced by the primer OPA8 on 30 of the plants tested. The presence of the 400-bp band is clearly evident in all male plants, as is its absence in female plants. Only in 2 cases out of 36 in cv , and in 1 case out of 22 in cv did a female plant show the OPA8400 marker;
Feminized
in monoecious plants, the RAPD band was always missing, at least in all of the samples we examined. In the other cultivars and accessions tested, the association of this band with the male phenotype was constant.
Hard to find this naming fact but the DNA sequences were named MADC1 and MADC2 (Male-Associated DNA from Cannabis sativa), according to the nomenclature in 1995. Sakamoto was one of the scientist on this project if you feel compelled to google.