Lets say you have a tote with 30 gallons of Nutrient water in it. If you have no airstones and no pump yet you leave the lid off, you will slowly develop of biofilm on the top of your water surface. If you put a small pump in the system, that does not cause surface agitation, and you leave the lid off, you will still get the biofilm on the entire surface this will stop all 02 from entering the water via surface gas exchange. If you only use an airstone you will get a biofilm but where the airbubbles come up and pop and make waves, they will be pushing the biofilm away. If you shut the stones off, the biofilm will quickly cover that water area that was bubbeling. This will actually increase the 02 levels of the water, even with the biofilm, just at the slowest possible level. Gas exchanges at the surface, except in the case of nanobubbles that can stay submerged for long periods of time. The gas is not exchanged while the bubble is traveling up the water, its more less increase the surface mixing when it pops, which increase gas exchange. Lets start with the first example again, if you only have a large pump, and your creating waves and you have no airstone, this will stop the biofilm from forming on the surface, but now it can stick on the side walls easier cause of the waves. Now if you add an airstone and a water pump, you are not only mixing the water and creating an even 02/nutrient water column, you are agitating the surface with the bubbles. Your creating small waves and then pulling the new molecules down. If you do not mix the water and it sits still, the o2 levels will decrease as you go deeper since gas is exchanged at the surface. A pump help create an even water column. A airstone with waterpump, waterfall, or ANY venturi, will all be beneficial. I choose the non pressurized venturi fitting but will eventually switch to the pressurized ones. I use it because I dont have to buy airstones now. There are alot of guys who legit just shove the airline in the res not attached to a stone or pump. Its pretty much how my 4 plant buckets work. I dont have enough pressure in them to create a venturi effect. But I also dont have to dig the airstone out of the roots, clean it only to find out its half clogged. Then there are waterfalls, which does basically the same thing. Waterfalls create surface agitation, keep an even o2 column, and there are no extra moving parts. If you go waterfalls you dont need a venturi fitting or airstones. I didnt like the waterfalls because im in love with venturis lol. . .and I dont even use them the correct way. Its just so much easier to put a pump in the res, and attach an airline to it. Im actually installing systems for others and if there was an easier way, id be doing it. Not saying easier is good for you, perhaps you should buy an 02 probe and collect your own data like moe. I do not lol