Milson
Milsonian
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Yeah man, the studies are mostly to tempt people into reading the whole paper as interested. Not needed.
I can sum up my hypothesis like this: it seems uvb excites receptors on uvr8, which influences the production of various proteins on the cellular level that lead to a variety of changes. It's plausible this is a mechanism that will increase the production of thc because thc absorbs uvb well and so can serve as "sunscreen."
Using tlc analysis, we can see if we can measure that effect in terms of thc, but also get some insight into how it influences thc (if it does) by looking at the data on other cannabinoids present in a tlc test and applying that to possible scenarios given the pathways observed by scientists for thc production.
I have also noted how these discoveries radically upset the working hypothesis of cannabinoid synthesis in play during much of the seminal research and academic work in the modern history of this plant's study.
Tldr: we are seeing what happens to a plant both chemically and visually when half of it is exposed to uv.
Well yes.. I mean would you be able to summarize what you are doing?
Yeah man, the studies are mostly to tempt people into reading the whole paper as interested. Not needed.
I can sum up my hypothesis like this: it seems uvb excites receptors on uvr8, which influences the production of various proteins on the cellular level that lead to a variety of changes. It's plausible this is a mechanism that will increase the production of thc because thc absorbs uvb well and so can serve as "sunscreen."
Using tlc analysis, we can see if we can measure that effect in terms of thc, but also get some insight into how it influences thc (if it does) by looking at the data on other cannabinoids present in a tlc test and applying that to possible scenarios given the pathways observed by scientists for thc production.
I have also noted how these discoveries radically upset the working hypothesis of cannabinoid synthesis in play during much of the seminal research and academic work in the modern history of this plant's study.
Tldr: we are seeing what happens to a plant both chemically and visually when half of it is exposed to uv.
To convert thca into thcWow this is so interesting - so the UV reduced the yield, but now you are going to analyze it for all the cannabinoids?
As a side note, I decarboxylate our trim and use it for edibles. Why do you decarbox before you test it?
It’s crazy about the colors. I wonder if the sun rays being different around the world makes them come out?
So many interesting things to learn about!
I need to convert the THCA to THC so that it can be properly detected by the test. I’ll show you more clearly in tomorrow’s TLC video.
Edit Aqua beat me to it
It's looking like a reduced yield is plausible, but i don't think we can say that for sure.Wow this is so interesting - so the UV reduced the yield, but now you are going to analyze it for all the cannabinoids?
As a side note, I decarboxylate our trim and use it for edibles. Why do you decarbox before you test it?
It’s crazy about the colors. I wonder if the sun rays being different around the world makes them come out?
So many interesting things to learn about!
That sound like a good plan. More time to recover in between. Could even do half hour 4 times.Maybe I should break it up and do 2 one hour bursts of UV separated by a few hours tomorrow?
I went ahead and did the 2 hours straight today by popular demand. Time to figure out tomorrow. With no other input I figure I’ll leave it a 2 but open to suggestionsThat sound like a good plan. More time to recover in between. Could even do half hour 4 times.
Agreed. I will only really be able to add to the anecdote pile because yield in weight is not a controlled test. But I’ll know personally because I can feel it. Just can’t prove it other than a pin disappearing.It's looking like a reduced yield is plausible, but i don't think we can say that for sure.
Then also the fact that it's plausible the slight reduction in size can be offset by a change in density. Often seen when grown under a more blue leaning spectrum tighter denser buds.Agreed. I will only really be able to add to the anecdote pile because yield in weight is not a controlled test. But I’ll know personally because I can feel it. Just can’t prove it other than a pin disappearing.
I'm thinking outside is always changing. Time of day, clouds and other shade... I have a uv that milson linked, but I haven't tried yet because they are pollinated and I don't want to hurt them. Maybe next time...I went ahead and did the 2 hours straight today by popular demand. Time to figure out tomorrow. With no other input I figure I’ll leave it a 2 but open to suggestions
Good news is I can change it at any time from my phone even when I’m not home so I can even decide tomorrow at work.
I totally agree with this. Whether it is operative and just how to test idk.I'm thinking outside is always changing. Time of day, clouds and other shade...
I think "where it pulls from" is a very complex question. I have seen studies that suggest se terpenes may be a precursor to olivolic acid, which starts the chain.
I really think I am losing terps in this process. Plants are still sticky AF, but the overpowering smell is gone.
I really noticed this last night when I opened my oven after decarb. Normally I get a face full of terpenes as the heat leaves the oven and I'm bending over to open it. My head is in the right (wrong) spot to get a good smell of whatever was cooking. I even comment on it in the early videos how strong it was. It's like a blast to the olfactory that is unmistakable.
Last night, meh.
And the smell in the tent is mostly gone. I have to get my nose directly on a bud to smell it.
Clearly not enough info to claim cause and effect, but it is not a small change. If terpenes are your shizzle, UV may not be right for you.
It is possible it is unrelated to UV completely. I don't really know, I'm just posting it up as an anomaly I did not really expect. If anything, I would have guessed an increase due to increase in trichomes we are expecting.Huh. Interesting. I had not considered nor ever read the effect on terpenes. Flavonoids too I imagine.
I have learned to move the plants away from the light a bit during ripening. Even a couple inches lower from the glass under the 600 hps’ last couple weeks seems to preserve more cannabinoids and terpenes for me.
It is possible it is unrelated to UV completely. I don't really know, I'm just posting it up as an anomaly I did not really expect. If anything, I would have guessed an increase due to increase in trichomes we are expecting.
Gotta figure out how to do scratch and sniff iPhones so you guys can smell the difference too.
I will say it has not been replaced by another smell like hay or something, it's just missing.
Non-scientific mention:
If this is real and UV diminishes terpenes and as it appears, reduces flower mass, those are 2 pretty big strikes against UV in my book. There have got to be some other benefits to this for UV to be a "success."
Tonight's TLC testing should give some answers. I'm testing 3 UV buds and 3 non-UV buds to try to get an average rather than a single data point (hat tip @Milson ). If cannabinoid proportions have changed between the test and control side, we will see it. If they have not, I will be looking at no upside but significant downside... I think. But it does seem to bring out the purple in Purple Kush... sort of a mixed bag so far.
Today is day 10, we are about 2/3 thru this test. 2 Hours today from 11:00 to 1:00.
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