Tricking plants into thinking there's an insect infestation, can it be done, and how...

[Frankster]

When I've had mites in the past, I'll have to admit, it was certainly some of the most dank, and special weed I've smoked over the years. Especially when I've had mites, and been able to successfully overcome them, by sheer nutrition, and general health. That said, I'm not eager to return to those days, but it get's me to thinking. What can be done... Obviously, the plants were aware of the onslaught, and were mounting a response. Especially during the flowering intervals.

So, I'm kind of intrigued by signaling hormones, precursors, enzymatic responses and other various means of inducing targeted signals to the plant. Wondering if anyone else out there has used any techniques in the past that are successful.

Please share anything you might have, or even your hypothesis on this subject. Putting some bugs up, since it's a "response" to bug thread...

 

[CurtisBlow916]

If heard of this and I’m interested in what others have to say.. From what I’ve read I think mites are actually the best type of pest to get, if you have to pick your poison.

 

[Frankster]

If heard of this and I’m interested in what others have to say.. From what I’ve read I think mites are actually the best type of pest to get, if you have to pick your poison.

Yea, I would tend to agree. Especially if your not talking broad/russet mites. Seems like even when I had some phytoseiulus persimilis and Amblyesius swirskii, (beneficial predatory mites) I was still producing much more sticky/tighter buds, so It makes me wonder if there's some some biological role at play here, that can be induced upon the plant.

 

[CurtisBlow916]

Yea, I would tend to agree. Especially if your not talking broad/russet mites. Seems like even when I had some phytoseiulus persimilis and Amblyesius swirskii, (beneficial predatory mites) I was still producing much more sticky/tighter buds, so It makes me wonder if there's some some biological role at play here, that can be induced upon the plant.

Woooow.. So basically any type of pest in the medium would trigger a reaction, CRAZY. Makes me wonder about insect frass, I know it has a way of tricking the plant into defending itself against pest that aren’t actually there so who knows

 

[Frankster]

Woooow.. So basically any type of pest in the medium would trigger a reaction, CRAZY. Makes me wonder about insect frass, I know it has a way of tricking the plant into defending itself against pest that aren’t actually there so who knows

Yea, that's a novel idea for sure. I'm actually using trelahose, which is a sugar that only insects make. Looks like trelahose is synthesized into trehalose 6-phosphate (t6p) by phosphate.

Trehalose, a sugar found in many species of plants and animals, is a non-reducing disaccharide composed of two glucose moieties. Its synthesis in plants is a two-step process, involving the production of trehalose-6-phosphate (T6P) catalyzed by trehalose-6-phosphate synthase (TPS) and its consecutive dephosphorylation to trehalose, catalyzed by trehalose-6-phosphate phosphatase (TPP). T6P has recently emerged as an important signaling metabolite, regulating carbon assimilation and sugar status in plants. In addition, T6P has also been demonstrated to play an essential role in plant development.

Trehalose is an ancient sugar consisting of two glucose molecules alpha-1,1-linked and thus with no reducing ends. It is one of only two nonreducing sugars found widely in nature, the other being sucrose. In bacteria, fungi, and insects, trehalose functions as a storage carbohydrate and protects against a variety of stresses. In plants, this role has been largely replaced by sucrose, although trehalose does protect against desiccation in certain specialized resurrection plants . Absence or trace amounts of trehalose in most plants precludes a role as a reserve or stress protectant.

 

[CurtisBlow916]

Yea, that's a novel idea for sure. I'm actually using trelahose, which is a sugar that only insects make. Looks like trelahose is synthesized into trehalose 6-phosphate (t6p) by phosphate.

Trehalose, a sugar found in many species of plants and animals, is a non-reducing disaccharide composed of two glucose moieties. Its synthesis in plants is a two-step process, involving the production of trehalose-6-phosphate (T6P) catalyzed by trehalose-6-phosphate synthase (TPS) and its consecutive dephosphorylation to trehalose, catalyzed by trehalose-6-phosphate phosphatase (TPP). T6P has recently emerged as an important signaling metabolite, regulating carbon assimilation and sugar status in plants. In addition, T6P has also been demonstrated to play an essential role in plant development.

Trehalose is an ancient sugar consisting of two glucose molecules alpha-1,1-linked and thus with no reducing ends. It is one of only two nonreducing sugars found widely in nature, the other being sucrose. In bacteria, fungi, and insects, trehalose functions as a storage carbohydrate and protects against a variety of stresses. In plants, this role has been largely replaced by sucrose, although trehalose does protect against desiccation in certain specialized resurrection plants . Absence or trace amounts of trehalose in most plants precludes a role as a reserve or stress protectant.

It’s a damn super sugar

 

[AZsunfarmer]

You could develop an mRNA mite vaccine to trigger the plants immune response. But it would be controversial and the forum would split into 2 different factions of pro/anti mite vaccine. Haha but anything antigenic that is specific to mites I suppose. I think you already mentioned in posts about watering that the dry cycle really stimulates a good stress response. It seems like indoor growing is really about not stressing the plant at all which seems contradictory to your hypothesis. How interesting!

 

[Lynch_Ironside]

very interesting thoughts indeed.
so when the plant is triggered by a pest and activates it’s immune response I assume there’s some specific response to each pathogen. And the response is triggered by Secretions in the pests’ saliva. So maybe there’s a way to synthesize spider mite saliva, or maybe there’s a way to extract whatever chemical compounds are heightened upon an attack from a sick plant, then inject these compounds into a healthy one. I would think you would need a laboratory for this but then again I saw a guy on YouTube extract dna in a shed behind his house. Full disclosure I read one article on this and that’s the extent of my knowledge, I’ll put a link below.
very cool idea though!

Mechanisms of plant defense against insect herbivores

Plants respond to herbivory through various morphological, biochemicals, and molecular mechanisms to counter/offset the effects of herbivore attack. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are ...

www.ncbi.nlm.nih.gov

 

[smokedareefer]

The Role of Botany and Plant Physiology in Cannabis Testing

Here we explore the role that terpenes play in plant immunity, defense, and signaling.

www.cannabissciencetech.com

 

[growsince79]

I once had a clone that never hermed until it got mites.

 

[Frankster]

You could develop an mRNA mite vaccine to trigger the plants immune response. But it would be controversial and the forum would split into 2 different factions of pro/anti mite vaccine. Haha but anything antigenic that is specific to mites I suppose. I think you already mentioned in posts about watering that the dry cycle really stimulates a good stress response. It seems like indoor growing is really about not stressing the plant at all which seems contradictory to your hypothesis. How interesting!

Yea, I think what's the key takeaway here is that (trehalose) a "bug" blood sugar is likely playing a key role in all of this... It's up taken into the plant and synthesized into trehalose 6-phosphate (t6p), which act's as an on/off switch for the associated stress respones.

I think there might be a dual pathway actually, but there's no question that the plants are picking up cues from bugs.

A procedure developed in 2017 which allows this sugar to be produced, but prior to that, it was exceptionally expensive and was only obtained though milking caterpillars. Now there's a pathway using corn starches.... Trehalose is a disaccharide formed by a 1,1-glycosidic bond between two α-glucose units so it's got a really cool spot in it's core that can transfer metal ions efficiently. This is especially important during flowering, and is key for calcium, phosphate and sulfur uptake. Note the center region where the oxygens have shared electron bonding....

 

[AZsunfarmer]

I know you are really invested into this prospect. Hopefully you share some data along the way because it’s so novel and fascinating but I understand not sharing too much if your hypothesis are indeed correct!

 

[Frankster]

I know you are really invested into this prospect. Hopefully you share some data along the way because it’s so novel and fascinating but I understand not sharing too much if your hypothesis are indeed correct!

Yea, no doubt there's multiple pathways of achieving these things, and there done to one degree to the next. It really is an interesting concept, and I've been picking apart a lot of the chemical process surrounding the flowering period these days, and specifically, for me, the interest is how our plants shift excess sugar production into oil production, and how those pathways are interlinked.

Both the trehalose and Sucrose biosynthesis pathways draw from a pool of core metabolites, from which the carbon skeletons for all cellular components are created. Flux through the trehalose pathway is more than a 1,000-fold lower than that through the Sucrose pathway. Were talking a tiny, tiny bit goes a long, long way.

 

[MrUnChained]

Just wondering where you locate this information? Looking to read into this.

 

[AnitaBonghitt]

I had a bad spider mite infection with 3 strains from clones. The chemdog did real well ,best its ever done. It looked real bad, the buds were small , but the buzz made it worth the trouble. I think with the chemdog, the worst it looks the better the smoke. I took cuts off the others ,but they just never came back and one even hermed on me.
Could be on to something here Frankster.

 

[Frankster]

Just wondering where you locate this information? Looking to read into this.

I've done lots of reading, but you can start here. This is all closely related to how phosphates, tp6, sugar and fatty acid's are replicated. See how t6p forms a circle transcribing sucrose and starches into hexose phosphates.... Off to the side, it's regulating growth, senescence, and ripening also... it's also got mechanisms for enhancing biotic and abiotic stress factors. It's the tale of two sugars.... Trehalose works at 1/100->1000th the quantities that sucrose are present. Although, I've been giving about 1/2 tsp per gal a few times a week now.

Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1

Trehalose 6-phosphate binds to the KIN10 subunit of SnRK1 and inhibits it in a GRIK-dependent manner, positively regulating fatty acid synthesis by stabili

academic.oup.com

Trehalose 6-phosphate phosphorylase - Wikipedia

en.wikipedia.org

Trehalose - Wikipedia

en.wikipedia.org

A Tale of Two Sugars: Trehalose 6-Phosphate and Sucrose

Trehalose 6-phosphate (), the intermediate of trehalose biosynthesis, is an essential signal metabolite in plants, linking growth and development to carbon status. The Suc-Tre6P nexus model postulates that is both a signal and negative feedback regulator ...

www.ncbi.nlm.nih.gov

Trehalose 6-phosphate signalling and impact on crop yield

The domestication and breeding of crops has been a major achievement for mankind enabling the development of stable societies and civilisation. Crops have become more productive per unit area of cultivated land over the course of domestication supporting a current global population of 7.8...

portlandpress.com

Multi-targeted trehalose-6-phosphate phosphatase I harbors a novel peroxisomal targeting signal 1 and is essential for flowering and development - Planta

Main conclusion This work reveals information about new peroxisomal targeting signals type 1 and identifies trehalose-6-phosphate phosphatase I as multitargeted and is implicated in plant development, reproduction, and stress response. Abstract A putative, non-canonical peroxisomal targeting...

link.springer.com

Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis

Fungal infections pose a serious threat to human health and result in several million deaths annually. To survive in their human host, pathogenic fungi require the disaccharide, trehalose. Significantly, the enzymes that synthesize trehalose are absent in humans, and thus serve as potential...

www.pnas.org

Frontiers | Trehalose-6-Phosphate: Connecting Plant Metabolism and Development

Beyond their metabolic roles, sugars can also act as messengers in signal transduction. Trehalose, a sugar found in many species of plants and animals, is a ...

www.frontiersin.org

Error - Cookies Turned Off

nph.onlinelibrary.wiley.com

Frontiers | Trehalose-6-Phosphate: Connecting Plant Metabolism and Development

Beyond their metabolic roles, sugars can also act as messengers in signal transduction. Trehalose, a sugar found in many species of plants and animals, is a ...

www.frontiersin.org

 

[Frankster]

This here part is a tightly related subject.

The regulation of trehalose metabolism in insects - PubMed

Trehalose is a non-reducing disaccharide comprising two glucose molecules. It is present in high concentration as the main haemolymph (blood) sugar in insects. The synthesis of trehalose in the fat body (an organ analogous in function to a combination of liver and adipose tissue in vertebrates)...

pubmed.ncbi.nlm.nih.gov

Insect trehalase: Physiological significance and potential applications

Abstract. Trehalose, a non-reducing disaccharide, is widespread throughout the biological world. It is the major blood sugar in insects playing a crucial r

academic.oup.com

The regulation of trehalose metabolism in insects - Cellular and Molecular Life Sciences

Trehalose is a non-reducing disaccharide comprising two glucose molecules. It is present in high concentration as the main haemolymph (blood) sugar in insects. The synthesis of trehalose in the fat body (an organ analogous in function to a combination of liver and adipose tissue in vertebrates)...

link.springer.com

Trehalose – The Insect ‘Blood’ Sugar

Download Citation | Trehalose – The Insect ‘Blood’ Sugar | Trehalose, the non-reducing disaccharide of glucose, is the principal sugar circulating in the blood or haemolymph of most insects. Resistance to... | Find, read and cite all the research you need on ResearchGate

www.researchgate.net

 

[Grapefruitroop]

Quitosan is what u guys are looking for!
For organic heads....Crab shell meal is a vey good source of it...

 

[CurtisBlow916]

Quitosan is what u guys are looking for!
For organic heads....Crab shell meal is a vey good source of it...

 

[ComfortablyNumb]

Ever hear of this?:
It tricks the plants into making more trichomes.

Sticky Icky-Enzyme Blend

Sticky Icky supplements your soil’s naturally produced enzymes, to give you a shortcut and a boost to radical oil and resin production.—including enzymes that “stress” your plants, sending them into oil-producing hyperdrive. This doesn’t hurt the plant...or your pocketbook, for that matter!

dakine420.com