Dr.Dutch
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Don't forget the nutrients. Only from sugar many bacteria can not grow.Next thing is to change growth medium and delivery. Start each in nute water and feed them sugar. Dextrose was this agar, but I'm thinking something mimicking Bud Candy would be a good next step.
Like plants and animals, they need other substances to build their DNA and divide.
With your plates you can just see which MOs grow only with sugar I would say.
Wiki also helps a bit.
https://en.wikipedia.org/wiki/Nutrient_agar
However, I think that using BudCandy is not a bad idea. You can say a lot of bad things about AN, but their fertilizer portfolio seems to make sense somehow.