Mushroom thread

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Swagmasterflex

Swagmasterflex

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I bought them this time, but I'm definitely going to start making them. People are proud of their agar 🤑 lol Hopefully I can find a PC at thrift store and then my ordering will be just for new genetics

So with no visible contamination on the dishes, do you always transfer just to another plate to verify or full send on it?
 
tobh

tobh

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I bought them this time, but I'm definitely going to start making them. People are proud of their agar 🤑 lol Hopefully I can find a PC at thrift store and then my ordering will be just for new genetics

So with no visible contamination on the dishes, do you always transfer just to verify or full send on it?
I'm damn near shiny new to agar. When I started growing many moons ago, agar work was reserved for the most advanced clandestine mycologists, so my input here is worth less than a grain of salt.

The idea is to start on agar with any new genetics, period. Print, spore syringe, LC syringe, don't trust em at all. Especially prints and spore syringes (I'm dealing with the proof of that logic now). Once you get a clean culture (no contamination, especially bacterial ime) then you can let the plate fully colonize, chop it up, make a LC or inoculate grains directly.

Since I've been getting deeper into it, as in having poured over 100 plates in the past months, I now understand why the idea of doing a grain jar inoculation with a spore syringe is a big no-no. Two of the syringes I ran last time I grew (a couple years ago) have done nothing but produced bacterial plates. The jars did the same thing. My aseptic technique is pretty solid, this isn't my first rodeo, and untouched plates haven't contamed, soooo....

Highly recommend getting a PC ASAP. If you can foot it, my current PC is a 24 quart Merro (T-Fal) I picked up for $80. I use a turkey fryer burner to heat it which I got for free. You can't do mycology without one, at least not easily -- I've done the ghetto style of boiling jars multiple days in a row with a cardboard box SAB. a PC of decent capacity changes everything and will pay for itself in months time.
 
Swagmasterflex

Swagmasterflex

79
33
I'm damn near shiny new to agar. When I started growing many moons ago, agar work was reserved for the most advanced clandestine mycologists, so my input here is worth less than a grain of salt.

The idea is to start on agar with any new genetics, period. Print, spore syringe, LC syringe, don't trust em at all. Especially prints and spore syringes (I'm dealing with the proof of that logic now). Once you get a clean culture (no contamination, especially bacterial ime) then you can let the plate fully colonize, chop it up, make a LC or inoculate grains directly.

Since I've been getting deeper into it, as in having poured over 100 plates in the past months, I now understand why the idea of doing a grain jar inoculation with a spore syringe is a big no-no. Two of the syringes I ran last time I grew (a couple years ago) have done nothing but produced bacterial plates. The jars did the same thing. My aseptic technique is pretty solid, this isn't my first rodeo, and untouched plates haven't contamed, soooo....

Highly recommend getting a PC ASAP. If you can foot it, my current PC is a 24 quart Merro (T-Fal) I picked up for $80. I use a turkey fryer burner to heat it which I got for free. You can't do mycology without one, at least not easily -- I've done the ghetto style of boiling jars multiple days in a row with a cardboard box SAB. a PC of decent capacity changes everything and will pay for itself in months time.
I definitely appreciate the advice! I did the same thing for shits and gigs and the pic is the end result lol! Just like everything cultivation; practice makes perfect. And I feel like a clandestine mycologist now lol

That's a killer Price, I'll definitely have to get one before I start the next round!
 
20221105 114203
Z

Zill

381
63
Hey,

I am coming into this conversation pretty late.

Back in graduate school myself and another graduate student got a hold of psilocybin shrooms. We filled about a dozen quart size mason jars with rye seeds. Rye seeds not pre-coated with fungicides. The filled mason jars were autoclaved and allowed to cool. Once cooled we blended the fruiting bodies with a little water to make flowable paste and pored equal amounts to each jar. We then capped the jars and set them on a shelf. After a period of time the tops were removed to induce fruiting bodies form. They never did. But the jars were full of beautiful hyphae that bruised blue. We cut the rye/hyphae mass out of the jar and ate the moldy rye seeds. The reaming was cut into bite size chunks, the into the freezer. It seems to retain its potency forever frozen.

Holy cow!! Forget mushrooms you don't need fruiting bodies to converse with the Masiah.

Zill.
 

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