Variety’s seed selecting and pollination thread

[Trustfall]

Yea, mine were more on the last run of Frankenstein, and some of the Early Miss Auto's. Don't know why it happened, or even if it actually did. But it was brought to my attention, and I noticed I was discarding half, maybe a little more than half of the plants. I think that last run I had like 21 and only 9 of them were females. No herms, but % in male ratio's could be a real thing, I suppose. I'm certainly not discounting that possibility, but I still think it's just luck of the draw.

But some others say they had worse ratio's. Not sure what to think. Cause male traits can be caused by not doing shit correctly, stress, ect... I just figured maybe the person had some stress issues, heat, ect. It was summertime in Az, so that got me wondering. Not exactly the most hospitable environment.

Stress IMO is a huge factor. Hell the wedding cake bx1’s I had two packs and there was 15 males!!! but fak me the god damn gnats got into them as soon as they sprouted and messed everything up. Now after I plant the seeds I spray the top of the dirt with DE and they can’t mess with them anymore.

 

[Trustfall]

That will make a few seeds

ya think? I kind of wonder how many pollens are actually in one of those bags.

I bet it's even more than what we imagine.

My guess is in nature >99%+ of that stuff ends up on the ground, or someplace it's not effective.

that dude really pumped it out there lol. My grape cream cake project was very hard to get pollen off the male I chose but then the wedding cake makes mother of gawd there was so much pollen in the tent I had to wipe it down about 20 times before I could get it all out. And I’m extremely allergic to it.

 

[Trustfall]

Here’s the other wedding cake bx1 I found. This one is pretty darn close to the cut I have.

 

[Frankster]

Stress IMO is a huge factor. Hell the wedding cake bx1’s I had two packs and there was 15 males!!! but fak me the god damn gnats got into them as soon as they sprouted and messed everything up. Now after I plant the seeds I spray the top of the dirt with DE and they can’t mess with them anymore.

Please be more specific here; your saying stress at the time of the sprouting? Or during any time....
I mean cannabis is certainly monoecious leaning; and some strains probably lean that direction more than others. ie. sativa's, and sativa leaning hybrids. So I tend to agree this is likely the case.

This is one I have a difficult time wrapping my head around what's actually happening here. How the plant is actually determining it's gender expression. ie. male; female; bi-sexual or monoecious.

I almost feel like there's 4 clear classifications here; but 2 of them are somewhat murky, and blend together, some overlap.

 

[Frankster]

ya think? I kind of wonder how many pollens are actually in one of those bags.

that dude really pumped it out there lol. My grape cream cake project was very hard to get pollen off the male I chose but then the wedding cake makes mother of gawd there was so much pollen in the tent I had to wipe it down about 20 times before I could get it all out. And I’m extremely allergic to it.

Yea, me too. My nose starts to itch right away, and I can tell pollens anywhere. Head tells me right away. Also such a talc type feel to it. It's pretty obvious if it's there in trace amounts, or not, even if you can't see it clearly, by feel. That silky touch.

 

[Trustfall]

Please be more specific here; your saying stress at the time of the sprouting? Or during any time....
I mean cannabis is certainly monoecious leaning; and some strains probably lean that direction more than others. ie. sativa's, and sativa leaning hybrids. So I tend to agree this is likely the case.

This is one I have a difficult time wrapping my head around what's actually happening here. How the plant is actually determining it's gender expression. ie. male; female; bi-sexual or monoecious.

I almost feel like there's 4 clear classifications here; but 2 of them are somewhat murky, and blend together, some overlap.

As you know I’m mot the science guy frank. Only go by what I see personally. I didn’t spray the top of the soil with DE after I planted them and the gnats were out of control elsewhere in the house and they hit them very early. Was my worst ratio to date.
And on the other hand the tk x gccf2’s were 4 females 6 males and they were safe from harm and at the same time the lemon tree x ibg99’s I got 7 females 3 males! so it’s all over the place so far.

 

[Trustfall]

Yea, me too. My nose starts to itch right away, and I can tell pollens anywhere. Head tells me right away. Also such a talc type feel to it. It's pretty obvious if it's there in trace amounts, or not, even if you can't see it clearly, by feel. That silky touch.

Can taste it too lol.

 

[Trustfall]

Speaking of tk x gccf2
This pheno has taken the lead.

And this one was neck and neck but has fallen back a bit.

But to be fair the second one doesn’t have as food of lighting.

 

[Trustfall]

This lemon tree x ibg99 starting to terp up and look at the spread on those fans!

 

[Frankster]

As you know I’m mot the science guy frank. Only go by what I see personally. I didn’t spray the top of the soil with DE after I planted them and the gnats were out of control elsewhere in the house and they hit them very early. Was my worst ratio to date.
And on the other hand the tk x gccf2’s were 4 females 6 males and they were safe from harm and at the same time the lemon tree x ibg99’s I got 7 females 3 males! so it’s all over the place so far.

Yea; it's difficult to say. Need to do widescale pops with lots of controls under varying conditions to tell for sure. Personally, I think if there skewing the ratio's it's done more on the female side and not from the actual male plant. ie. Another females skews the ratio's. That's my belief for what it's worth. The rest is totally random numbers; like throwing dice; or playing roulette.

He's a little blurb I'm reading now; on a related subject.

It is predominantly dioecious, meaning a plant is either a male or a female, with estimated haploid genome sizes of 843 Mb and 818 Mb for male and female plants, respectively (Van Bakel et al., 2011). Despite the presence of defined sex chromosomes, environmental factors such as reduced photoperiod and low temperature, and foliar applications of chemicals such as silver nitrate and the ethylene hormone inhibitor silver thiosulfate induce pollen production in female flowers, leading to the production of ‘feminised seeds’ (Ram & Sett, 1982; Kaushal, 2012; Lubell & Brand, 2018). This technique has been exploited as a useful tool in cannabis breeding (for example, selfing or crossing female plants) and in generating populations for dissection of the genetic bases of important traits.

So 843mb - 818mb = 25 mb or about 2.965% of the plant DNA are written for maleness. ie. <3%

Precursor synthesis of these cannabinoids occurs from two distinct metabolic pathways; the polyketide pathway and the methylerythritol phosphate (MEP) pathway (Fig. 1) (Kovalchuk et al., 2020). These produce alkylresorcinolic acids, including olivetolic acid (OA) that is specific to cannabis, and geranyl diphosphate (GPP), respectively. CBGA (cannabigerolic acid) is then synthesized from OA and GPP to produce the acidic precursors of THC (tetrahydrocannabinolic acid; THCA) and CBD (cannabidiolic acid; CBDA)

That pathway in the yellow section there; is primarily created though microbial activity; it seems. I'm trying to read up on methods of how to increase genetic complexity, and spur the plant to evolve and differentiate itself. Not it's not a switch, but a parallel pathway. I really think cannabis is far more capable of evolving than currently understood; I would argue it's mostly driven by conditions. ie. diet, microbes, environmental stimulus.

https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.17140

 

[Frankster]

This lemon tree x ibg99 starting to terp up and look at the spread on those fans!

What the heck; lemon tree x ibg99; (lemon tree x Big bud x C99?) It looks very sativa leaning there. Those fans look almost as thin and long as the wild Thai.

 

[Trustfall]

What the heck; lemon tree x ibg99; (lemon tree x Big bud x C99?) It looks very sativa leaning there. Those fans look almost as thin and long as the wild Thai.

Yo Frank! That’s the lemon tree clone x (Indiana bubblegum xc99) . I pollinated the bubblegum with c99 then found a male when I popped a few of those. Then pollinated the dudded lemon tree.
The c99 puts off pine and pine apple phenos of whatever I pollinated with out. The long finger fans come from the pineapple side of the c99 the pines are smaller.
here is a pick from the first page of this thread of donkey butter x c99 pineapple leaners. Some of the od xc99 had some really nice fans as well.
But back to the lemon tree x ibg99 that’s probably the best looking fans so far.

 

[Madmax]

You got me sitting up paying attention to that pic lol...ive just put toilet roll round my neck to catch all the slobber coming out haha.

 

[Frankster]

Yo Frank! That’s the lemon tree clone x (Indiana bubblegum xc99) . I pollinated the bubblegum with c99 then found a male when I popped a few of those. Then pollinated the dudded lemon tree.
The c99 puts off pine and pine apple phenos of whatever I pollinated with out. The long finger fans come from the pineapple side of the c99 the pines are smaller.
here is a pick from the first page of this thread of donkey butter x c99 pineapple leaners. Some of the od xc99 had some really nice fans as well.
But back to the lemon tree x ibg99 that’s probably the best looking fans so far.

Love it brother, those fine looking giraffe heads and that frost, those pistols are looking very
Looks like a vertical frost factory.

What ever your doing to drive this part is where your really nailing it well. Simply Amazing. What's that spray your using? You think that's a part of what's magnify your frost factor?

 

[Trustfall]

Love it brother, those fine looking giraffe heads and that frost, those pistols are looking very
Looks like a vertical frost factory.

What ever your doing to drive this part is where your really nailing it well. Simply Amazing. What's that spray your using? You think that's a part of what's magnify your frost factor?

It’s the only reason I’m still in business Frank… the looks, the terps and the high’s is why they come back. You won’t get the same at the dispensary unless your paying 3-400$ an ounce.
Heavy 16 Foilar is a fantastic product. Here’s a link to better understand what it does then I can tell you. Scroll pass the price tag for the info.

Heavy 16 Foliar | Purchase Heavy 16 Foliar Spray to Fortify Plants | HTG Supply

Push plants to their true potential with Heavy Foliar (1-0-2) by Heavy 16! Heavy 16 Professional Foliar Nutrient (PFN), "Foliar" for short, is designed to mitigate stress and fortify your plants. Heavy Foliar will give you peace of mind when dealing with plant stress and provide your plants...

www.htgsupply.com

In my terms it makes the fan leaves bigger. Everything else has to be going right as well to really see the benefits. They recommended spraying till day 28 of flower but I will NOT spray anything on my plants after week one.
another benefit is pest like mites and thrips really do not like it.

 

[Trustfall]

Tootsie everything is a bit smaller this round which sucks but the quality
Is up.
after switching from silicium to powersi the power si sunk my ph. Took a ton of up to get it back in range and I noticed after about 5-6 days the plants were getting pissed and dropped it from the lineup. Still killed a growth week

 

[Trustfall]

This is how I collected the pollen on the lemon tree x ibg99 he gave a lot.

And this female is going to get some pollen to make f2’s and see if I can bump out the Cindy phenos or at least lower the count.
Not the best growing plant, kind of sucks like granddaddy purple but the terps are loud. Not pollinating until next round though want to smoke it to see what’s up first.

 

[Trustfall]

Maybe these will be a little more exciting. Don’t judge them to hard as they are in the worst lighting spots in the room.
3 phenos of triangle kush x grape cream cake f2.
This looks like it will be the heaviest yielder.

and this one is a grape leaner

And my favorite right now a triangle kush leaner

 

[Madmax]

This is how I collected the pollen on the lemon tree x ibg99 he gave a lot.View attachment 1192059View attachment 1192060
And this female is going to get some pollen to make f2’s and see if I can bump out the Cindy phenos or at least lower the count.
Not the best growing plant, kind of sucks like granddaddy purple but the terps are loud. Not pollinating until next round though want to smoke it to see what’s up first.

LOL....howd you bend that stem without snapping it..looks kinda freaky ...

 

[Trustfall]

LOL....howd you bend that stem without snapping it..looks kinda freaky ...

Haha pretty tough stem I must say! He kept drinking like that too before I cut him off.