As you know I’m mot the science guy frank. Only go by what I see personally. I didn’t spray the top of the soil with DE after I planted them and the gnats were out of control elsewhere in the house and they hit them very early. Was my worst ratio to date.
And on the other hand the tk x gccf2’s were 4 females 6 males
and they were safe from harm and at the same time the lemon tree x ibg99’s I got 7 females 3 males!
so it’s all over the place so far.
Yea; it's difficult to say. Need to do widescale pops with lots of controls under varying conditions to tell for sure. Personally, I think if there skewing the ratio's it's done more on the female side and not from the
actual male plant. ie. Another females skews the ratio's. That's my belief for what it's worth. The rest is totally random numbers; like throwing dice; or playing roulette.
He's a little blurb I'm reading now; on a related subject.
It is predominantly dioecious, meaning a plant is either a male or a female, with estimated haploid genome sizes of 843 Mb and 818 Mb for male and female plants, respectively (Van Bakel et al., 2011). Despite the presence of defined sex chromosomes, environmental factors such as reduced photoperiod and low temperature, and foliar applications of chemicals such as silver nitrate and the ethylene hormone inhibitor silver thiosulfate induce pollen production in female flowers, leading to the production of ‘feminised seeds’ (Ram & Sett, 1982; Kaushal, 2012; Lubell & Brand, 2018). This technique has been exploited as a useful tool in cannabis breeding (for example, selfing or crossing female plants) and in generating populations for dissection of the genetic bases of important traits.
So 843mb - 818mb = 25 mb or about 2.965% of the plant DNA are written for maleness. ie. <3%
Precursor synthesis of these cannabinoids occurs from two distinct metabolic pathways; the polyketide pathway and the methylerythritol phosphate (MEP) pathway (Fig. 1) (Kovalchuk et al., 2020). These produce alkylresorcinolic acids, including olivetolic acid (OA) that is specific to cannabis, and geranyl diphosphate (GPP), respectively. CBGA (cannabigerolic acid) is then synthesized from OA and GPP to produce the acidic precursors of THC (tetrahydrocannabinolic acid; THCA) and CBD (cannabidiolic acid; CBDA)
That pathway in the yellow section there; is primarily created though microbial activity; it seems. I'm trying to read up on methods of how to increase genetic complexity, and spur the plant to evolve and differentiate itself. Not it's not a switch, but a parallel pathway. I really think cannabis is far more capable of evolving than currently understood; I would argue it's mostly driven by conditions. ie. diet, microbes, environmental stimulus.