Which is the most reliable seed bank?

  • Thread starter Valenjulio
  • Start date
  • Tagged users None
Deadstill

Deadstill

On Vacation
Supporter
2,739
263
Buzzy12 said:
So is there anyway to check for this virus outside of buying an expensive device to test for it? Phenohunting high quality packs of seeds seems more important than ever. The 10% bleach solution sprayed on seeds sounds like a good idea. A 10% hydrogen peroxide solution would probably achieve the same goal.

Not sure how this virus is going to be stopped at this point. This is an interesting article I found. This excerpt is particularly concerning considering most estimates are that about 80% of the market currently is GSC hybrids.

"Interestingly, the review article also notes that only a few cannabis cultivars actually show symptoms associated with HLVd when infected, which implies that the disease severity and symptoms could be genotype-dependent. Although, as the review authors also point out, there is no current evidence of any HLVd-resistant cultivars."

www.analyticalcannabis.com

Hop Latent Viroid is a Major Threat to the Cannabis Industry, Review Claims

The review details the discovery of HLVd in hops and subsequently in the cannabis plant, provides a brief overview of the extent of these infections in the industry, and offers guidance on the prevention and management of infections.
www.analyticalcannabis.com www.analyticalcannabis.com
Click to expand...
So is there anyway to check for this virus outside of buying an expensive device to test for it?
Click to expand...
No. And you have to send samples to a qualified lab to get positive identification. They will run a LAMP or PCR test to detect it. The best and most accurate part of the plant to send in for testing is the roots. Test any other part of the plant, and it does not have 100% success in detection.

The 10% bleach solution sprayed on seeds sounds like a good idea. A 10% hydrogen peroxide solution would probably achieve the same goal.
Click to expand...
No, it will not. Hydrogen peroxide or alcohol will not work as a substitute and can actually spread it more.

I highly recommend these folks at Kaycha Labs for HVL'd testing - https://www.kaychalabs.com/
 
Buzzy12

Buzzy12

Supporter
672
143
Deadstill said:
I highly recommend these folks at Kaycha Labs for HVL'd testing - https://www.kaychalabs.com/
Click to expand...
I think the real question here is how much do we really need to worry about this virus? This virus could have been present in cultivars as far back as the 80's or even earlier. They first discovered the virus on hops plants in the 80's. The only reason we know it exists now is because we have the ability to test for it. It's nicknamed the "dud plant" disease. In the past if you had a dud plant, you just got rid of it...

That article I shared is enlightening. A lot of cultivars are going to be resistant to this virus. Only the strong survive. Phenohunting and keeping things clean are vitally important. This does not seem like a concern for homegrowers at all. It's much more of a concern for these facilities that are way bigger than they need to be where quality control is an issue. That's just my two cents being a small professional grower that hates corporate America.

Deadstill said:
No, it will not. Hydrogen peroxide or alcohol will not work as a substitute and can actually spread it more
Click to expand...
I figured hydrogen peroxide is an excellent alternative to bleach and would work the same way. Do you have any information on why it would help spread it more? Liquid oxygen is excellent for seeds as well to help give the initial tap root a source of energy right away. I had that recommended to me earlier this year, it made sense, I tried it, and it works really well. 10% hydrogen peroxide solution is what I used.
 
Trustfall

Trustfall

Supporter
5,898
313
Deadstill said:
No. And you have to send samples to a qualified lab to get positive identification. They will run a LAMP or PCR test to detect it. The best and most accurate part of the plant to send in for testing is the roots. Test any other part of the plant, and it does not have 100% success in detection.


No, it will not. Hydrogen peroxide or alcohol will not work as a substitute and can actually spread it more.

I highly recommend these folks at Kaycha Labs for HVL'd testing - https://www.kaychalabs.com/
Click to expand...
Don’t forget to mask 😷 up, social distance, and wash your hands for 30 seconds before rinsing 🧼 🧽 🤪
And send me all your special cuts and I’ll pcr test them for you!
IMG 9457
 
Captspaulding

Captspaulding

What’s the matter? Don’t like clowns? 🤡
Staff
Supporter
22,233
638
Glassdub said:
There is a zillion cloning threads on the naked THCFarmer site yet this one is called "Which is the most reliable seed bank"
I don't piss in your pool... 🥹
Click to expand...
Ok man, you’re weird. I’m out of this dumpster fire 😂😂😂
 
Deadstill

Deadstill

On Vacation
Supporter
2,739
263
Deadstill said:
I highly recommend these folks at Kaycha Labs for HVL'd testing - https://www.kaychalabs.com/
Click to expand...

I'm so sorry I accidentally posted the wrong lab. I meant to say Tumi Genomics - https://tumigenomics.com/hop-latent-viroid-testing
Buzzy12 said:
I figured hydrogen peroxide is an excellent alternative to bleach and would work the same way. Do you have any information on why it would help spread it more? Liquid oxygen is excellent for seeds as well to help give the initial tap root a source of energy right away. I had that recommended to me earlier this year, it made sense, I tried it, and it works really well. 10% hydrogen peroxide solution is what I used.
Click to expand...

The problem is alcohol and hydrogen peroxide need more time to kill viruses. It can take up to 5 minutes for hydrogen peroxide to effectively kill viruses. Alcohol is a little faster, but the problem is they lose effectiveness quickly, not killing all of the virus, and actually tend to spread it around, more.

I attended a seminar by Dr. Tassa Saldi of Tumi Genomics at last year's CannaCon, which is how I know all of this. I took a lot of notes.

But if you search around there is a lot of documentation on why bleach is more effective at eliminating viruses than other methods.

Here's a doc on detection - https://21668670.fs1.hubspotusercon...s/Hop Latent Viroid/HLVd Validation-Final.pdf

From their FAQ section - While alcohol can be effective to sterilize against many pathogens, it is NOT effective at removing viroid contamination. Alcohol actually stabilizes viroids, which increases the rate of transmission between plants. Bleach can leave a salt residue after drying and alcohol can be used to remove this residue. However, care should be taken to NOT mix liquid bleach and alcohol, as this makes a toxic gas (chloroform). Alcohol can also be used to remove plant matter before bleaching as long as a bleach step is included after.

As I understand it, from everything I have read and been told about HLV'd, is that prevention is much cheaper and easier than mitigation. In other words it's easier/better/cheaper to take the extra precautions to prevent getting HLV'd, than it is to remove an infection from a facility.

Buzzy12 said:
I think the real question here is how much do we really need to worry about this virus? This virus could have been present in cultivars as far back as the 80's or even earlier. They first discovered the virus on hops plants in the 80's. The only reason we know it exists now is because we have the ability to test for it. It's nicknamed the "dud plant" disease. In the past if you had a dud plant, you just got rid of it...
Click to expand...



Personally I think the reason why it hasn't been seen as often as it is, now, is simply due to legalization. Up until the past 10 years or so, there haven't been many facilities growing cannabis to notice it. Now, it is estimated that over 75% of all facilities across the country have HLV'd.
 
Toohighmyguy

Toohighmyguy

225
63
That dude 😎 was serious 🧐 bout his seeds. And his 💩 says all you fellow growmies 👾👾👾. Were out of line ➖. Will someone give this dude 😡 a reputable seed 🏦. I just wanted to say I thought 🤔 all the girls 🪴🌲🌴🌿🍀 were beautiful 😍. 🤣🤣🤣🤣🤣🤣🤣
 
GNick55

GNick55

Staff
Supporter
13,396
438
Deadstill said:
No. And you have to send samples to a qualified lab to get positive identification. They will run a LAMP or PCR test to detect it. The best and most accurate part of the plant to send in for testing is the roots. Test any other part of the plant, and it does not have 100% success in detection.


No, it will not. Hydrogen peroxide or alcohol will not work as a substitute and can actually spread it more.

I highly recommend these folks at Kaycha Labs for HVL'd testing - https://www.kaychalabs.com/
Click to expand...
it can miss detection in the roots also..
 
Trustfall

Trustfall

Supporter
5,898
313
It’s easier to blame viroids for shitty grow practices than to actually hold yourself accountable for growing problems.
And deadstill I recently heard that tumi is going back on the root test being the most accurate. Everything you heard a year ago probably all different now . 😁
 
GNick55

GNick55

Staff
Supporter
13,396
438
Trustfall said:
It’s easier to blame viroids for shitty grow practices than to actually hold yourself accountable for growing problems.
And deadstill I recently heard that tumi is going back on the root test with being the most accurate. Everything you heard a year ago probably all different now . 😁
Click to expand...
who’s blaming hlvd?????
most accurate but not perfect.. still it’s a waste of money testing..
 
GNick55

GNick55

Staff
Supporter
13,396
438
Trustfall said:
The entire cannabis commercial industry 😅
We have our own pcr tester here at my work and we are required to test weekly before we take cuts.
Click to expand...
i hear ya..
it’s a terrible issue..
i’m just freaked out about it being so infectious to so many grow operations especially the breeding projects for so long that it has gotten deep into the industry worldwide with seeds and clones, with that how many seedbanks etc have seed stock that is infected.. all the growers that still don’t know and who breed or clone and pass along.. it’s well known more than half the weed in dispensaries are infected, than all the people who buy it find seeds and grow them etc etc….
 
You must log in or register to reply here.

Latest posts

Top Bottom