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We have a half dozen positives for TMV in cannabis, immuno-assays. Fairly unreliable imo. They're for toba genus. We also have maybe 25 solid duds cases testing negative by the same method. Lots of work ongoing on this now. It's all serology and PCR based. It'll be a long study. We'll publish...

Yeah our data set is pretty extensive. It's infectious, 100%. I don't have data pre-2008. @WeSolidarity suggests root aphids possibly vectored this originally. This is consistent with our data. Here's an example of alarming trend:

Yup. @WeSolidarity is right. We're pretty far past "cut better clones." Our research team is building a new lab in the Arcata, CA Marijuana Innovation Zone in northern California, called Humboldt DNA. We're specifically going to perform the PCR work necessary to ID the viruses (it's likely more...

Papa, Some guy actually says: if your plants are duds, cull them and replace them with ones that aren't visibly diseased. Hard to argue with that. If they're your only plants, run them through see what you get and get better ones next time. Don't panic, just grow the best plants you can. If you...

Sounds dudsy for sure. No treatment, just cull and try to outgrow infection. GG4 is particularly susceptible

Read pages 27+ of this thread. GG4 is lovely

I observe stem discolorations yellow, red and in severe cases, brownish. Always brittle. If you can reach over and snap a plant with minimal torque, it's toast. I get what your going for. Visual ID would be useful. I suspect everything other than the most healthy green stemmed individuals. If...

We're doing a ton of work on this in Northern CA. I'd bet all the money that the industry is inundated with multiple viral infections in many if not all states. Seeds are not immune as this is very likely pollen borne. There's no solution or fixing infected plants. Identifying symptomatic...

We're there ever any duds in the genetics you're reveging? Was there even one from that stock that you culled or set aside before you flowered it? Or do you believe it's never had a dud? What strain?

Vaccinations don't work in plants as far as I know. We needs tests. This will take work to figure out. If it's like strawberries, it's spread already. Everything pretty much has it. If you've seen it, it's there. Plant herpes that only becomes symptomatic if the host is particularly susceptible...

It's troubling that people growing from seed are also seeing plenty of duds. I don't know if that means it's pollen borne, but that seems unfortunately likely. Hopefully the meristem isn't carrying virus and it's cleanable. As far as milk goes, even if it worked as a sort of control, it has to...

This is pretty much a must-watch for anybody following this thread closely. The Q&A at the end is worth the wait. All of it lands pretty much on the money. 45 minutes well spent.

No those look like cells differentiating to form callus, then roots, but they hit air and light so they stopped. CaC2O4 crystals are microscopic but not real small. Maybe 12-25μm

Autoclave or pressure cooker

There are so many questions to be answered here, I don't know. I'll be operational in weeks, results will take months. If this is viral, TC is the most viable solution. Heat therapy looks promising, sort of. If you're set up to do that, run it and let us know how you do. It's tough to do it...

That's what led me to look at root zone fungal infections to start. I pulled my hair out looking for a fungus - for maybe two years - something that causes duds. This started because of the absence of anchor roots in clones and plants. So the generic dwarfing and runting of the plants appears to...

I also do not see dudding

Yeah the crystal idioblasts themselves aren't remarkable. It's the quantity not the presence that I find interesting. Nothing conclusive, but I've examined a ton of samples and my observations are consistent enough to publicly speculate about why they're showing up. Heavily infected plants tend...

Solid analysis. I bought RNA Pro for science to see what they'd send me. It was a plastic jar of misc white crystalline powder that smells sort of sweet and acidic. Smells like aspirin. The label is computer paper and poorly affixed. The directions indicate one scoop per 50 gallons. There was no...

That's our next step. I've got a culture lab for in-house fungal diagnostics but that's easy compared to this. Meristem tissue culture hasn't been necessary for me until now. It's tough. It'll take a few months to get operational but we've pulled the trigger. I'll update. I still want an ID...