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That's very interesting because I just saw online where they took spinach leaves, removed the plant matter to leave cellulose and implanted heart cells into it. They had it beating in 5 days. Apparently the human vascular system is closely related to plants structure. Cool stuff!
Totally agree, sometimes it's easier and more cost effective to work with what you got as opposed to trying to fight mother nature. Next week I'll have to check out that nano breathe, seems like something suitable for my situation. Getting tight being close to the holidays and all. Went a little crazy on a genetics auction lol, just bid on and won two auctions. Some purple pez, and a grab bag of a bunch of random packs of beans. 40 total. Got a good deal and went to a good cause so I'm more than happy with it.
Thank you, I've finally dialed in my cloning technique and have been able to keep them healthy from the time I cut them at flip to when my room frees up and I can start pumping them hard. Basically just growing roots until I pot them 2-3 weeks before I harvest the main room. That's all I want them to do at that point, grow roots.
Bacillus Amyloliquefaciens is the bacteria in Hydroguard, supposedly they live in bigger, more vigorous colonies than Subtilis, therefore are able to survive more unfavorable conditions. Idk if that's just marketing? Seemed to help the bio film in my cloner res.
There are a lot of bacteria and enzyme products out, makes it hard to determine which are necessary, useful, or useless. SLF-100 is a product that has huge following, I had some samples and it appears to break down the bone meal gum, but I don't really know if it's worth the price. I found some information on the product (unofficially from the owner, Newton Hayes, but due to legalities and/or labeling laws, I'm unsure why, but I don't think he was able to publish it. I say unofficial bc I found it on the Nectar for the Gods website) Reading that .pdf you posted on PSBs and their role in plant growth promotion, it made me feel slow to learn Bacillus can be PSB. :smoking:
Part of a Q&A with the owner of South Cascade Organics - Newton Hayes -
In your own words Newton, what is SLF-100?
“I want to post what SLF-100 is rather than a bunch of hype. That way, you can do the research
if you want. SLF stands for Submerged Liquid Fermentation. SLF is a microbe based enzymatic
product. We start with a base of microbes which we add to several species of grasses that we
grow. We then bring in plants from extreme environments, for their ability to survive those
extremes, and we let it ferment. Most of the plants we bring in are proprietary but one plant
comes from a salt flat for the plants ability to survive high EC's. From this plant we get bacteria
like Virgibacillus pantothenticus which is known to help plants survive osmotic stress and
produces enzymes necessary to remediate high salt environments. SLF-100 was actually created
to break down salts. Breaking phosphorus bonds and calcium build up (bone gum) are beneficial
side effects from the use of SLF-100. It is not just a microbial product. There are microbes left
from the fermentation process and researching the specific microbes will help to understand what
the product is capable of.
SLF-100 is: A formulation of multiple species of bacteria, however there are four that really
stand out and make their presence known.
• Pseudomonas putida - Phosphor solubilizer - amazing bacteria, also an antagonist for
pythium and fusarium.
• Virgibacillus pantothenticus - production of ectoine and proline, causes plants to adapt to
environmental stressors - among many other things
• Bacillus thuringiensis - helps with some pests - also has anti-pathogenic properties.
• Bacillus subtillus - catalase-positive bacteria - meaning given the ability to grow
overnight in a simple sugar medium and added to H2O2 it will break the extra oxygen
atom off the end - the real enzyme test.”
~ Newton Hayes
Seems like a pretty straight forward and honest guy. I emailed him about trying it and he didn't even respond.. which was kinda weird.. he just sent me 2 small bottles to try. I emailed again to thank and he did respond that time. Here's the original .pdf file if interested - https://nectarmerch.com/collections/digital-downloads/products/slf-100-users-guide
Is this the ideal way to introduce these strains of bacteria?
Sounds Ok to me, and most of the stuff sold contains lots of microbes which are otherwise padding if you like, Literally it would be impossible to make a plant tonic which is specific to a single plant, without sampling every single plant on the planet and in all conditions, but this is in fact the reality in the wild.
Some plants have lots of Biological Primer Partners (BPP), some hardly any. Japanese hogweed has about 20, where Sunflowers about 4. The more BPP relationships you can form, the wider your chance of acquiring what you need at any one time. The plants with lower ratios of BPP's must have conditions that favor these partners, or there will be trouble.
Bac Am is in Bio Media Pro, I have been using it for many years now, in fact since about 1990 I think. Most of the stuff we now use for plant growth promotion and bio control, came from our past/ existing business which was green waste management and mining restoration. This I did before I decided to become a full time farmer, and still do on a consultancy basis.
I make an SLF type of liquid inoculate, called K+AMINO. It is made in a very similar way, except we live in areas of extremes deliberately and so rarely have to ship in, rather taking samples from the wild and then growing these on in situ where we then collect living samples as the base to further process internally using the same basic principles for KNF fermentation.
K+AMINO is rammed full of PSB's KSB. SRB's and Nitrogen fixers. it is highly loaded with bio controls, esp valuable for pre treating land after winter, where negative biology can gain traction amid the cool damp of winter and early spring. If you needed an inoculate for single season plants, this is a really good option. Not just because we make it, but because it can consolidate many of the individual products we might otherwise buy, and it can be used across plant types, including legumes. it is rich in siderophore producing microbes, some of those are in the SLF-100 above I can see.
I use K+AMINO as a foliar at 0.01% from seed up to week 3-4 bloom typically on MJ. It is an excellent seed soak too, containing many microbes that are germination antagonists, working to chew the gums from seeds cases, rhizobial as well as autotrophs working more generally on soil health to restrict common white molds, fusarium and so on.
It also contain trichoderma, both viride and hazarium strains so boith N fixing and Bio Control. We only harvest microbes from the wild, we dont buy in spores for onward cultivation.
Why do we only attempt to use wild microbes and not simply get them from a lab which would be super easy?
Most of the knowledge about Bacillus subtilis derives from studies of laboratory strains growing as planktonic cultures, in which all the individual cells are considered identical. Recently, the study of a natural and undomesticated isolate has revealed that B. subtilis cells display multicellular and social features that were lost in the laboratory strains, which were selected over generations for easy manipulation. In undomesticated strains, certain environmental conditions trigger cells of this bacterium to form multicellular communities where sporulation takes place, and to exhibit some particular social traits, like swarming motility, the fratricide of sibling cells or cannibalism during sporulation, and the release of extracellular DNA. Interestingly, some of these behaviors are based in the heterogeneity of the B. subtilis populations, which has been determined using cell biological techniques like fluorescence and light microscopy. - https://www.caister.com/bacillus2017
:)